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Influence of hormones on DNA synthesis of breast tumors in culture

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Abstract

Tissue culture techniques have been developed for studying the influence of hormones on human breast tissues. The present study demonstrated that estrogen induced a highly significant increase in3H-thymidine incorporation into DNA and labelling index of ductal epithelium of fibrocystic disease; there was no effect of progesterone, either alone or in combination with ovine prolactin, on benign lesions. Estrogen stimulated certain malignant tumors derived from postmenopausal women. These studies also showed that there was an inhibitory effect of3H-thymidine incorporation into DNA by the effect of progesterone on malignant lesions. When menopausal status was considered, it was found that DNA synthesis was significantly higher in the presence of insulin and hydrocortisone in malignant tumors derived from premenopausal women than from postmenopausal women, or than in benign lesions. Thus, the present findings may provide evidence that specific activity may be an important measurement for breast tumor DNA synthesis in response to ovarian hormones or other substances.

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Authors and Affiliations

  1. Departamento de Biología, Universidad Metropolitana de Ciencias de la Educacion, Casilla 147 D, Santiago, Chile

    Gloria Calaf

  2. Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile

    Fernando Garrido

  3. Facultad de Medicina, Universidad de Chile, Santiago, Chile

    Carlos Moyano & Roberto Rodríguez

Authors
  1. Gloria Calaf
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  2. Fernando Garrido
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  3. Carlos Moyano
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  4. Roberto Rodríguez
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Calaf, G., Garrido, F., Moyano, C. et al. Influence of hormones on DNA synthesis of breast tumors in culture. Breast Cancer Res Tr 8, 223–232 (1986). https://doi.org/10.1007/BF01807335

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