Cultures of exfoliated mammary epithelial cells: Variation between donors
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Human milk contains clonogenic epithelial cells which give rise to three distinct colony types, namely, joined elongated, joined cuboidal, and open (i.e. unjoined). Previous studies have shown that these may be interrelated and may reflect stages in cell differentiation in the human mammary gland. This paper reports an analysis of paired milk samples from a series of donors, and shows that the predominant clonogenic cell type varies from donor to donor. This could reflect individual variation in the cell population of the lactating human breast.
Keywordsbreast epithelium clonal types donor variation milk cell culture
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- 1.Metcalf D: Regulation by colony stimulating factor of granulocyte and macrophage colony formation in vitro by normal and leukaemic cells.In Clarkson B, Baserga R (eds): Control of Proliferation in Animal Cells. Cold Spring Harbor, 1974, pp 887–905Google Scholar
- 3.Greaves MF, Rao J, Hariri G, Verbi W, Catowski D, Kung PJ, Goldstein G: Phenotypic heterogeneity and cellular origins of T cell malignancies. Leukaemia Res 5: 281–299, 1980Google Scholar
- 6.Stoker M, Perryman M, Eeles R: Clonal analysis of morphological phenotype in cultured mammary epithelial cells from human milk. Proc R Soc Lond B 215: 213–240, 1980Google Scholar
- 8.Stoker M: The untransformed cell. J Cell Physiol (Suppl) 2: 11–18, 1982Google Scholar
- 10.Bennett DC, Peachey LA, Durbin H, Rudland PJ: A possible mammary stem cell line. Cell 15: 283–298, 1980Google Scholar