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Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors

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Summary

A transplantable pregnancy-dependent mouse mammary tumor, TPDMT-4, and its ovarian-dependent (T4-OR26) and autonomous (T4-OI96, T4-OI145, T4-OI165, T4-OI320 and T4-OI320CY) sublines were examined immunohistochemically for the expression of keratin 14 and type IV collagen. T4-OI96, T4-OI145, and T4-OI165, but not T4-OR26, T4-OI320, or T4-OI320CY, formed lung colonies (metastasis) after intravenous injection as a single-cell suspension. Despite the similar morphology of TPDMT-4 and its six sublines, only TPDMT-4 and the nonmetastatic sublines revealed a basal cell phenotype as defined by keratin 14 expression. Staining for type IV collagen was complete at the peripheries of the glandular structures in TPDMT-4 and nonmetastatic sublines but was patchy in the metastatic tumors.

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Authors and Affiliations

  1. Department of Pathology, Kansai Medical University, Moriguchi, 570, Osaka, Japan

    Airo Tsubura, Toshihiko Inui & Sotokichi Morii

  2. Lawrence Berkeley Laboratory, 1 Cyclotron Road, 94720, Berkeley, CA, USA

    Shahnaz Hashmi Dairkee

  3. Division of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, 3–18 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan

    Tsutomu Oikawa

  4. Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, 108, Tokyo, Japan

    Akio Matsuzawa

Authors
  1. Airo Tsubura
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  2. Toshihiko Inui
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  3. Sotokichi Morii
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  4. Shahnaz Hashmi Dairkee
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  5. Tsutomu Oikawa
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  6. Akio Matsuzawa
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Tsubura, A., Inui, T., Morii, S. et al. Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors. Breast Cancer Res Tr 17, 239–243 (1991). https://doi.org/10.1007/BF01806373

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  • DOI: https://doi.org/10.1007/BF01806373

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