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Polypeptide composition of normal and neoplastic human breast tissues and cells analyzed by two-dimensional gel electrophoresis

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Summary

The protein populations of epithelial cells cultured from two neoplastic and five non-neoplastic human breast tissues were resolved and displayed by two-dimensional polyacrylamide gel electrophoresis and silverstaining. With a computer-based image analysis system, we identified eight polypeptides which are present in both of the neoplastic cell lines, but absent from all five of the cultures of non-neoplastic breast cells. The eight polypeptides are not unique to cells cultured from neoplastic breast, because they are also found in cells cultured from non-breast tissues, both neoplastic and non-neoplastic. Two of the eight polypeptides (∼ Mr 25,000/pI 4.4 and ∼ Mr 31,000/pI 5.5) are present in the patterns of whole tissue samples from infiltrating ductal carcinomas and absent in most normal breast tissue.

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Authors and Affiliations

  1. Department of Research Administration, Michigan Cancer Foundation, 110 E. Warren Avenue, 48201, Detroit, MI, USA

    Terry M. Maloney

  2. Department of Biological Sciences, St. John's University, 11439, Jamaica, New York, USA

    Philip L. Paine

  3. Department of Pathology, Michigan Cancer Foundation, 110 E. Warren Avenue, 48201, Detroit, Michigan, USA

    Jose Russo

Authors
  1. Terry M. Maloney
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  2. Philip L. Paine
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  3. Jose Russo
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Maloney, T.M., Paine, P.L. & Russo, J. Polypeptide composition of normal and neoplastic human breast tissues and cells analyzed by two-dimensional gel electrophoresis. Breast Cancer Res Tr 14, 337–348 (1989). https://doi.org/10.1007/BF01806306

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