Skip to main content
SpringerLink
Log in

Heterogeneity of estrogen binding sites in mammary tumors

  • Scandinavian Breast Cancer Symposium
  • Published:
Breast Cancer Research and Treatment Aims and scope Submit manuscript

Summary

This paper provides a brief summary of our recent work on the heterogeneity of estradiol binding sites in mammary tumors of the mouse and human. Mammary tumors from both species contain the classical estrogen receptor and a second site(s) which we call type II. These type II sites are found in the cytoplasmic and nuclear compartments of both tumors, and can interfere with the measurement of estrogen receptors. The function of type II sites is not known; however, an assessment of these sites in conjunction with an accurate measurement of the estrogen receptor may provide a clearer picture of the true hormone dependence of breast tumors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Clark JH, Peck EJ Jr: Female Sex Steroids: Receptors and Function. Springer-Verlag, Berlin, 1979

    Google Scholar 

  2. Eriksson H, Upchurch S, Hardin JW, Peck EJ Jr, Clark JH: Heterogeneity of estrogen receptors in the cytosol and nuclear fractions of the rat uterus. Biochem Biophys Res Commun 81:1–7, 1978

    PubMed  Google Scholar 

  3. Clark JH, Hardin JW, Upchurch S, Eriksson H: Heterogeneity of estrogen binding sites in the cytosol of the rat uterus. J Biol Chem 253:7630–7634, 1978

    PubMed  Google Scholar 

  4. Watson CS, Clark JH: Heterogeneity of estrogen binding sites in mouse mammary cancer. J Recep Res 1:91–111, 1980

    Google Scholar 

  5. Scatchard G: The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51:600–672, 1949

    Google Scholar 

  6. Panko WB, Watson CS, Clark JH: The presence of a second specific estrogen binding site in human breast cancer. J Steroid Biochem 14:1311–1316, 1981

    PubMed  Google Scholar 

  7. Syne JS, Markaverich BM, Clark JH, Panko WB: Estrogen binding sites in the nucleus of normal and malignant human tissue: I: Optimization of an exchange assay for the measurement of specific binding. Cancer Research 42:4443–4448, 1982

    PubMed  Google Scholar 

  8. Rosenthal HE: A graphic method for the determination and presentation of binding parameters in a complex system. Anal Biochem 20:525–532, 1967

    PubMed  Google Scholar 

  9. Feldman HA: Mathematical theory of complex ligandbinding system of equilibrium: some methods for parameter fitting. Anal Biochem 48:317–338, 1972

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, Baylor College of Medicine, Houston, Texas, USA

    J. H. Clark, C. S. Watson, B. M. Markaverich, J. S. Syne & W. B. Panko

Authors
  1. J. H. Clark
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. S. Watson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. M. Markaverich
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. S. Syne
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. B. Panko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Clark, J.H., Watson, C.S., Markaverich, B.M. et al. Heterogeneity of estrogen binding sites in mammary tumors. Breast Cancer Res Tr 3, 61–65 (1983). https://doi.org/10.1007/BF01806234

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01806234

Keywords

Search

Navigation