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Metabolism of kinetin by soybean callus

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Abstract

Soybean callus metabolised applied 6-furfurylamino (8-14C) purine very rapidly to polar compounds, some of which were retained on a Dowex 50 cation exchange resin, and were unaffected by alkaline phosphatase; while others were apparently phosphorylated and were detected in the aqueous fraction. Adenine was detected as an intermediate and it can be concluded that it was formed as a result of the rapid and efficient removal of the furfuryl side chain. The formed adenine was rapidly incorporated into the polar metabolites. Exactly how the presence of this cytokinin stimulates cell division is not apparent from the results.

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References

  1. ArmstrongDJ, BurrowsPK and SkoogF (1969) Isolation of cytokinins from tRNA. Biochem Biophys Res Commu 37: 415–456

    Google Scholar 

  2. El-SaidiMT (1971) Transport and metabolism of kinetin-8-14C inZea mays L. roots. Ann Bot 35: 1073–1083

    Google Scholar 

  3. GzikA and GüntherG (1982) Der Metabolismus von {8-14C}-Kinetin inBeta vulgaris (Zuckerrübe) undChenopodium album (Weisser Gänsefus). Biochem Physiol Pflanzen 177: 395–402

    Google Scholar 

  4. HuttonMJ and VanStadenJ (1981) An efficient colum chromatographic method for separating cytokinins. Ann Bot 47: 527–529

    Google Scholar 

  5. LethamDS (1978) Cytokinins. In: DSLetham, PBGollnow and TJVHiggins, eds. Phytohormones and Related Compounds: A Comprehensive Treatise I, pp 205–263. Amsterdam: Elsevier/North-Holland

    Google Scholar 

  6. MiernykJA and BlaydesDF (1977) Short-term metabolism of radioactive kinetin during lettuce seed germination. Physiol Plant 39: 4–8

    Google Scholar 

  7. MillerCO (1965) Evidence for the natural occurrence of zeatin and derivatives: Compounds from maize which promote cell division. Proc Natl Acad Sci USA 54: 1052–1058

    PubMed  Google Scholar 

  8. MillerCO (1968) Naturally-occurring cytokinins. In: FWightman and GSetterfield, eds. Biochemistry and Physiology of Plant Growth Substances, pp. 33–45. Ottawa: The Runge Press Ltd

    Google Scholar 

  9. VanStadenJ (1982) Transport and metabolism of 8{14C} zeatin in dormant plane cuttings during cold storage. Z Pflanzenphysiol 105: 351–357

    Google Scholar 

  10. VanStadenJ (1982/1983) The metabolism of 6-(2,3,4-trihydroxy-3-methylbutylamino) purine by soybean callus. Pl Growth Regul 1: 253–260

    Google Scholar 

  11. VanStadenJ (1983a) Short term metabolism of different concentrations of zeatin applied to soybean callus. Z Pflanzenphysiol 109: 163–169

    Google Scholar 

  12. VanStadenJ (1983b) Long term metabolism of zeatin supplied to soybean callus. Z Pflanzenphysiol 111: 423–428

    Google Scholar 

  13. VanStadenJ and DaveyJE (1977) The metabolism of zeatin and zeatin riboside by soya bean callus. Ann Bot 41: 1041–1048

    Google Scholar 

  14. VanStadenJ and HuttonMJ (1982) Metabolism of 8{14C}t-zeatin in soybean callus. Z Pflanzenphysiol 106: 355–354

    Google Scholar 

  15. VanStadenJ, WebbDP and WareingPF (1972) The effect of stratification on endogenous cytokinin levels in seeds ofAcer saccharum. Planta 104: 110–114

    Google Scholar 

  16. VeenH and JacobsWP (1969) Movement and metabolism of kinetin-14C and of adenine-14C inColeus petiole segments of increasing age. Plant Physiol 44: 1277–1284

    Google Scholar 

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The financial support of the C.S.I.R., Pretoria, and The Natal University Development Fund is gratefully acknowledged.

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Van Staden, J., Forsyth, C. Metabolism of kinetin by soybean callus. Plant Growth Regul 3, 167–178 (1985). https://doi.org/10.1007/BF01806056

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