Breast Cancer Research and Treatment

, Volume 14, Issue 1, pp 101–105 | Cite as

Histone acetylation decreased by estradiol in the MCF-7 human mammary cancer cell line

  • J. R. Pasqualini
  • P. Mercat
  • N. Giambiagi
Brief communication


The effect of estradiol (E2) on the [3H]-acetylation of nuclear histones was studied in the MCF-7 human mammary cancer cell line in culture. Cells (~ 108) were incubated with 8 × 10−6M [3H]-acetate in the absence (control) or in the presence of estradiol (10−5–10−8M). After 20 min incubation, the nuclear histones were extracted and separated by electrophoresis, and each histone band was measured and calculated in DPM/mg protein. It was observed that only the H2 + H3 and H4 histones were associated with the [3H]-acetate. Estradiol (10−6–10−8M) provoked a significant inhibition in the incorporation of the acetate. The negative effect, in percentage of the non-treated cell value, was as follows: in E2 (10−6M): − 25 ± 10 (SE) for H2 + H3 and − 26 ± 5 for H4; in E2 (10−7M): − 35 ± 9 and − 39 ± 10; and in E2 (10−8M): − 56 ± 22 and − 30 ± 13 respectively. It is concluded that estradiol has a negative effect in the acetylation of H2, H3 and H4 histones of this mammary cancer cell; no acetylation or effect is observed in H1 histones. The relationship of this finding to the biological responses of the hormone is to be explored.

Key words

human mammary cancer cells MCF-7 nuclear histones estradiol acetylation 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • J. R. Pasqualini
    • 1
  • P. Mercat
    • 1
  • N. Giambiagi
    • 1
  1. 1.C.N.R.S. Steroid Hormone Research UnitFoundation for Hormone ResearchParisFrance

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