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Human placental steroid sulphatase—purification and monospecific antibody production in rabbits

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Journal of Inherited Metabolic Disease

Abstract

Human steroid sulphatase was purified 43-fold from placental microsomes using a four step procedure: solubilization with Miranol H2M, Bio-Gel A 1.5m chromatography, column chromatofocusing and Sephadex G-75 chromatography. The purified enzyme that appeared electrophoretically homogeneous was used to immunize rabbits. Protein blotting demonstrated that the resulting antiserum mainly reacted with a polypeptide of 63 000 dalton, which is about the size of placental steroid sulphatase. The antiserum was freed from minor impurities by absorbing it to Sepharose 4B with immobilized antigens prepared from a steroid sulphatase deficient placenta.

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References

  • Andrews, P. Molecular sieve chromatography applied to molecular size and molecular weight estimation.Lab. Pract. 16 (1967) 851–855

    Google Scholar 

  • Burns, G. R. J. Purification and partial characterization of arylsulphatase C from human placental microsomes.Biochem. Biophys. Acta 759 (1983) 199–204

    PubMed  Google Scholar 

  • Crawfurd, M. d'A. Review: Genetics of steroid sulphatase deficiency and X-linked ichthyosis.J. Inher. Metab. Dis. 5 (1982) 153–163

    PubMed  Google Scholar 

  • Dancis, J., Jansen, V. and Hutzler, J. Hair root analysis in X-linked ichthyosis.J. Inher. Metab. Dis. 6 (1983) 173–177

    PubMed  Google Scholar 

  • France, J. T. Steroid sulphatase deficiency.J. Steroid Biochem. 11 (1979) 647–651

    PubMed  Google Scholar 

  • France, J. T. and Liggins, G. C. Placental sulphatase deficiencyJ. Clin. Endocrinol. Metab. 29 (1969) 138–141

    PubMed  Google Scholar 

  • Gauthier, R., Vigneault, N., Bleau, G., Chapdelaine, A. and Roberts, K. D. Solubilization and partial purification of steroid sulphatase of human placenta.Steroids 31 (1978) 783–798

    PubMed  Google Scholar 

  • Harkness, R. A. Review: Current clinical problems in placental steroid of arylsulphatase C deficiency and related “cervical dystocia” and X-linked ichthyosis.J. Inher. Metab. Dis. 5 (1982) 142–144

    PubMed  Google Scholar 

  • Harkness, R. A., Taylor, N. F., Crawfurd, M. A. and Rose, F. A. Recognizing placental steroid sulphatase deficiency.Br. Med. J. 287 (1983) 2–3

    Google Scholar 

  • Iwamori, M., Moser, H. W. and Kishimoto, Y. Solubilization and partial purification of steroid sulphatase from rat liver: characterization of estrone sulphatase.Arch. Biochem. Biophys. 174 (1976) 199–208

    PubMed  Google Scholar 

  • Jöbsis, A. C., van Duuren, C. Y., de Vries, G. P., Koppe, J. C., Ryken, Y., van Kempen, C. M. J. and de Groot, W. P. Trophoblast sulphatase deficiency associated with X-linked chromosomal ichthyosis.Ned. Tijdschr. Geneesk. 120 (1976) 1980

    Google Scholar 

  • Jöbsis, A. C., de Groot, W. P., Tigges, A. J., de Bruijn, H. W. A., Ryken, Y., Meijer, A. E. F. H. and Marinkovic-Ilsen, A. X-linked ichthyosis and X-linked placental sulphatase deficiency: A disease entity.Am. J. Pathol. 99 (1980) 279–290

    PubMed  Google Scholar 

  • Jöbsis, A. C., van der Loos, C. M., Walboomers, J. M. M. and de Groot, W. P. Letter to the editor.Br. Med. J. 288 (1984) 233–234

    Google Scholar 

  • Jöbsis, A. C., de Vries, G. P., Meijer, A. E. F. H. and Ploem, J. S. The immunohistochemical detection of prostatic acid phosphatase: Its possibilities and limitations in tumor histochemistry.Histochem. J. 13 (1981) 961–973

    PubMed  Google Scholar 

  • Koppe, J. G., Marinkovic-Ilsen, A., Ryken, Y., de Groot, W. P. and Jöbsis, A. C. X-Linked ichthyosis: A sulphatase deficiency.Arch. Dis. Child. 53 (1978) 803–806

    PubMed  Google Scholar 

  • Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227 (1970) 680–685

    PubMed  Google Scholar 

  • van der Loos, C. M., van Breda, A. J., van den Berg, F. M. and Jöbsis, A. C. The nature of placental steroid sulphatase deficiency in man.J. Steroid Biochem. 18 (1983) 1743–1746

    Google Scholar 

  • van der Loos, C. M., van Breda, A. J., Meijer, A. E. F. H. and Jöbsis, A. C. Biochemical investigation to the reliability of the histochemical demonstration of arylsulphatase activity in cryostat sections.Histochemistry 73 (1981) 161–164

    PubMed  Google Scholar 

  • van der Loos, C. M., Volkers, H., Tigges, A. J., Meijer, A. E. F. H. and Jöbsis, A. C. Combined histochemical and biochemical investigation to the reliability of the demonstration of arylsulphatase activity in cryostat sections.Histochemistry 62 (1979) 259–269

    PubMed  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J. and Farr, A. L. Protein measurement with the folin phenol reagent.J. Biol. Chem. 193 (1951) 265–275

    PubMed  Google Scholar 

  • McKee, J. W. A. and France, J. T. Studies of the biochemical basis of steroid sulphatase deficiency—III. Phospholipid composition of microsomes from normal and steroid sulphatase deficient placentas.J. Steroid. Biochem. 18 (1982) 309–312

    Google Scholar 

  • McNaught, R. W. and France, J. T. Studies on the biochemical basis of steroid sulphatase deficiency: preliminary evidence suggesting a defect in membrane-enzyme structure.J. Steroid Biochem. 13 (1980) 363–373

    PubMed  Google Scholar 

  • Meyer, J. Ch., Grundmann, H. P. and Schnyder, U. W. Determination of arylsulfatase C in hair follicles.Arch. Dermatol. Res. 266 (1979) 95–97

    PubMed  Google Scholar 

  • Migeon, B. R., Shapiro, L. J., Norum, R. A., Mohandas, T., Axelman, J. and Dabora, R. L. Differential expression of steroid sulphatase locus on active and inactive human X chromosome.Nature 299 (1982) 838–840

    PubMed  Google Scholar 

  • Moriyasu, M., Ito, A. and Omura, T. Purification and properties of arylsulphatase C from rat liver microsomes.J. Biochem. 92 (1982) 1189–1195

    PubMed  Google Scholar 

  • Rose, F. A. Review: The mammalian sulphatase and placental sulphatase deficiency in man.J. Inher. Metab. Dis. 5 (1982) 145–152

    PubMed  Google Scholar 

  • Shapiro, L. J., Weiss, R., Webster, D. and France, J. T. X-linked ichthyosis due to steroid sulphatase deficiency.Lancet 1 (1978) 70–72

    PubMed  Google Scholar 

  • Traupe, H. and Happle, R. Clinical spectrum of steroid sulphatase deficiency: X-linked ichthyosis, birth complications and cryptorchidism.Eur. J. Pediatr. 140 (1983) 19–21

    PubMed  Google Scholar 

  • Vaessen, R. T. M. J., Kreike, J. and Groot, G. S. P. Protein transfer to nitrocellulose filters—A simple method for quantitation a single protein in complex mixtures.FEBS Lett. 124 (1981) 193–196

    PubMed  Google Scholar 

  • Williams, M. L., Grayson, S., Bonifas, J. M., Epstein, E. H. and Elias, P. M. Epidermal cholesterol sulphate and steroid sulphatase activity and recessive X-linked ichthyosis. In Marks, R. and Plewid, O. (eds.)The Stratum Corneum, Springer, Berlin, 1983, pp. 79–85

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Pathology, Academical Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands

    C. M. van der Loos, A. J. van Breda, F. M. van den Berg, J. M. M. Walboomers & A. C. Jöbsis

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  1. C. M. van der Loos
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  2. A. J. van Breda
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  3. F. M. van den Berg
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  4. J. M. M. Walboomers
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  5. A. C. Jöbsis
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van der Loos, C.M., van Breda, A.J., van den Berg, F.M. et al. Human placental steroid sulphatase—purification and monospecific antibody production in rabbits. J Inherit Metab Dis 7, 97–103 (1984). https://doi.org/10.1007/BF01801762

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  • DOI: https://doi.org/10.1007/BF01801762

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