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Journal of Inherited Metabolic Disease

, Volume 11, Issue 3, pp 319–323 | Cite as

E2-cDNA cloning and component X of pyruvate dehydrogenase complex

  • S. Matuda
  • K. Nakano
  • I. Tabata
  • S. Matuo
  • T. Saheki
Jsimd Meeting
  • 19 Downloads

Keywords

Public Health Internal Medicine Pyruvate Metabolic Disease Pyruvate Dehydrogenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cederbaum, S. O., Blass, J. P., Minkoff, N., Brown, J., Cotton, M. E. and Harris, S. H. Sensitivity to carbohydrate in a patient with familial intermittent lactic acidosis and pyruvate dehydrogenase deficiency.Pediatr. Res. 10 (1976) 713–720Google Scholar
  2. DeMarcucci, O. L. and Lindsay, J. G. Component X. An immunologically distinct polypeptide associated with mammalian pyruvate dehydrogenase multi-enzyme complex.Eur. J. Biochem. 149 (1985) 641–648Google Scholar
  3. DeMarcucci, O. L., Hunter, A. and Lindsay, J. G. Low immunogenicity of the common lipoamide dehydrogenase subunit (E3) of mammalian pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multienzyme complexes.Biochem. J. 226 (1985) 509–517Google Scholar
  4. Ho, L., Hu, C. W-C., Packman, S. and Patel, M. S. Deficiency of the pyruvate dehydrogenase component in pyruvate dehydrogenase complex-deficient human fibroblasts.J. Clin. Invest. 78 (1986) 844–847Google Scholar
  5. Jilka, M. J., Rahmatullah, M., Kazemi, M. and Roche, E. T. Properties of a newly characterized protein of the bovine kidney pyruvate dehydrogenase complex.J. Biol. Chem. 261 (1986) 1858–1867Google Scholar
  6. Kresze, G-B. and Ronft, H. Bovine kidney pyruvate dehydrogenase complex. Limited proteolysis and molecular structure of the lipoate acetyltransferase component.Eur. J. Biochem. 112 (1980) 589–599Google Scholar
  7. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T-4.Nature (London) 227 (1970) 680–685Google Scholar
  8. Linn, T. C., Pelly, J. W., Pettit, F. H., Hucho, H., Randall, D. D. and Reed, L. J. α-Keto acid dehydrogenase complexes. XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart.Arch. Biochem. Biophys. 148 (1972) 327–342Google Scholar
  9. Maniatis, T., Fritsch, E. F. and Sambrook, T. Hybridization to nitrocellulose filters containing replicas of bacteriophage plaques or bacterial colonies. InMolecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982, pp. 326–328Google Scholar
  10. Matuda, S., Shirahama, T., Saheki, T., Miura, S. and Mori, M. Purification and immunochemical studies of pyruvate dehydrogenase complex from rat heart, and cell-free synthesis of lipoamide dehydrogenase, a component of the complex.Biochim. Biophys. Acta 741 (1983) 86–93Google Scholar
  11. Matuda, S., Nakano, K., Matuo, S. and Saheki, T. Molecular cloning of cDNA for rat liver lipoate acetyltransferase, a component of pyruvate dehydrogenase complex.Biochem. Biophys. Res. Commun. 142 (1987) 953–957Google Scholar
  12. Rahmatullah, M. and Roche, E. T. The catalytic requirements for reduction and acetylation of protein X and the related regulation of various forms of resolved pyruvate dehydrogenase kinase.J. Biol. Chem. 262 (1987) 10265–10271Google Scholar
  13. Raymond, Y. and Shore, G. C. The precursor for carbamyl phosphate synthetase is transported to mitochondria via a cystosolic route.J. Biol. Chem. 254 (1979) 9335–9338Google Scholar
  14. Weber, K. and Osborn, M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.J. Biol. Chem. 244 (1969) 4406–4412Google Scholar

Copyright information

© SSIEM and MTP Press Limited 1988

Authors and Affiliations

  • S. Matuda
    • 1
  • K. Nakano
    • 2
  • I. Tabata
    • 1
  • S. Matuo
    • 3
  • T. Saheki
    • 3
  1. 1.Department of BiologyKanoya National Institute of Fitness and SportsKanoyaJapan
  2. 2.Department of BiochemistryKagoshima Women's Junior CollegeKagoshimaJapan
  3. 3.Department of Biochemistry, School of MedicineKagoshima UniversityKagoshimaJapan

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