Skip to main content
Log in

Modulation of platelet Ca2+ homeostasis by hypertensive plasma factor(s) derived from patients with early-stage renal disease

Klinische Wochenschrift Aims and scope Submit manuscript

Summary

To determine whether blood-borne factors in hypertension accompanying early-stage kidney disease might be responsible for altered cellular calcium homeostasis, we measured changes in cytosolic calcium before and after incubating platelets in plasma ultrafiltrates from normotensive and hypertensive renal patients. With the use of the chelating agent quin 2, we found the free-calcium concentrations in platelets to be higher in the hypertensive than in the normotensive group. When both groups of participants were combined, a direct correlation was found between arterial pressure and cytosolic calcium. The cytosolic calcium concentration in platelets of normotensive renal patients increased after incubation with plasma from patients with untreated renal hypertension, but it was unchanged after incubation with plasma from normotensive subjects. These data indicate that the total cell burden of calcium is increased in platelets of hypertensive patients with earlystage renal disease, and that plasma from these patients contains a substance that is capable of increasing the cytosolic calcium concentration in platelets. If the plasma factor (or factors) acts not only on platelets, but also on vascular smooth muscle cells, it may contribute to the increased peripheral vascular resistance associated with hypertension of renal origin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Astarie C, Levenson J, Simon A, Meyer P, Devynck MA (1989) Platelet cytosolic proton and free calcium concentrations in essential hypertension. J Hypertens 7:485–491

    Google Scholar 

  2. Aviv A, Livne A (1988) The Na+ /H+ antiport, cytosolic free calcium, and essential hypertension: a hypothesis. Am J Hypertens 1:410–413

    Google Scholar 

  3. Bachmann J, Schlüter H, Storkebaum W, Witzel H, Wessels F, Zidek W (1991) Effects of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney. Clin Sci 80:17–23

    Google Scholar 

  4. Beretta-Piccoli C, Weidmann P, Schiffl H, Cottier C, Reubi FC (1982) Enhanced cardiovascular pressor reactivity to norepinephrine in mild renal parenchymal disease. Kidney Int 22:297–303

    Google Scholar 

  5. Blaustein MP (1977) Sodium ions, calcium ions, blood pressure regulation and hypertension. A reassessment and a hypothesis. Am J Physiol 231:C165-C173

    Google Scholar 

  6. Brickman AS, Nyby MD, von Hungen K, Eggena P, Tuck ML (1990) Calcitropic hormones, platelet calcium, and blood pressure in essential hypertension. Hypertension 16:515–522

    Google Scholar 

  7. Brod J, Bahlmann J, Cachovan M, Hubrich W, Hundeshagen H (1980) Pathogenese des renalen Hochdruckes. Klin Wochenschr 58:719–726

    Google Scholar 

  8. Brod J, Schaeffer J, Hengstenberg H, Kleinschmidt TG (1984) Investigations on the Na+, K+ pump in erythrocytes of patients with renal hypertension. Clin Sci 66:351–355

    Google Scholar 

  9. Chandra M, Rastogi R, Kumar A, Misra R, Shukla RN, Saxena AK, Shanker K (1990) Platelet ionic calcium and serum total calcium in essential hypertension. Indian J Exp Biol 28:96–97

    Google Scholar 

  10. Da Prada M, Zürcher G (1976) Simultaneous radioenzymatic determinations of plasma and tissue adrenaline, noradrenaline, and dopamine within femtomole range. Life Sci 19:1161–1174

    Google Scholar 

  11. Davidson WD, Sackner MA (1963) Simplification of the anthrone method for the determination of inulin in clearance studies. J Lab Clin Med 62:351–356

    Google Scholar 

  12. De Wardener HE, MacGregor GA (1980) Dahl's hypothesis that a saluretic substance may be responsible for a sustained rise in arterial pressure: its possible role in essential hypertension. Kidney Int 18:1–19

    Google Scholar 

  13. Erne P, Bolli P, Burgisser E, Bühler FR (1984) Correlation of platelet calcium with blood pressure: effect of antihypertensive therapy. N Engl J Med 310:1084–1088

    Google Scholar 

  14. Fröhlich ED, Tarazi RC, Dustan HP (1969) Re-examination of the hemodynamics of hypertension. Am J Med Sci 257:9–23

    Google Scholar 

  15. Grollman A, Krishnamurty VSR (1971) A new pressor agent of renal origin: its differentiation from renin and angiotensin. Am J Physiol 221:1499–1506

    Google Scholar 

  16. Haber E, Koerner T, Page KB, Kliman B, Purnode A (1969) Application of radioimmunoassay for the angiotensin I to the physiologic measurements of plasma renin activity in normal subjects. J Clin Endocrinol 29:1349–1355

    Google Scholar 

  17. Haller H, Oeney T, Huck U, Distler A, Philipp T (1989) Increased intracellular free calcium and sensitivity to angiotensin II in platelets of pre-eclamptic women. Am J Hypertens 2:238–243

    Google Scholar 

  18. Kilby MD, Broughton Pipkin F, Cockbill S, Heptinstall S, Symonds EM (1990) A cross-sectional study of basal platelet intracellular free calcium concentration in normotensive and hypertensive primigravid pregnancies. Clin Sci 78:75–80

    Google Scholar 

  19. Kramer HJ, Heppe M, Penning J, Knipowski J, Klingmüller D, Düsing R, Krück F (1985) Relation of endogenous digoxin-like immunoreactive activities to salt balance and renal function in man. Klin Wochenschr 62 [Suppl 3]:107–110

    Google Scholar 

  20. Land K, Kjeldsen SE, Od I, Westheim A, Hjermann I, Eide T, Gjesdal K (1988) Increased platelet and vascular smooth muscle reactivity to low dose adrenaline infusion in mild essential hypertension. J Hypertens 6:219–225

    Google Scholar 

  21. Le Quan Sang KH, Pernollet MG, Meyer P, Devynck MA (1990) Plasma digitalis-like activity and cytosolic calcium in essential hypertension. Am J Hypertens 3:171–175

    Google Scholar 

  22. Levenson J, Devynck MA, Pithois-Merli I, Le Quan Sang KH, Filitti V, Simon A (1990) Dynamic association between artery shear flow condition and platelet cytosolic free Ca2+ concentration in human hypertension. Clin Sci 79:613–618

    Google Scholar 

  23. Lindner A, Kenny M, Meacham AJ (1987) Effects of a circulating factor in patients with essential hypertension on intracellular free calcium in normal platelets. N Engl J Med 316:509–513

    Google Scholar 

  24. Marone C, Beretta-Piccoli C, Weidmann P (1980) Acute hypercalcemic hypertension in man: role of hemodynamics, catecholamines and renin. Kidney Int 20:92–96

    Google Scholar 

  25. Matsukawa S, Suzuki H, Jtaya Y, Nakajima S, Saruta T (1987) Short- and long-term efficacy of nifedipine in hypertensive patients with impaired renal function, with special reference to influencing factors. J Clin Hypertens 3:452–462

    Google Scholar 

  26. Michelakis AM, Mizukoshi H, Huang C, Murakami K, Inagami T (1975) Further studies on the existence of a sensitizing factor to pressure agents in hypertension. J Clin Endocrinol Metab 41:90–96

    Google Scholar 

  27. Mizukoshi H, Michelakis AM (1972) Evidence for the existence of a sensitizing factor to pressor agents in the plasma of hypertensive patients. J Clin Endocrinol Metab 34:1016–1024

    Google Scholar 

  28. Norris KC, Nemen MN, Kert CJ, Muallem S, Levine BS (1990) Basal and stimulated cytosolic platelet calcium in essential hypertension. J Hum Hypertens 4:110–112

    Google Scholar 

  29. Pritchard K, Raine AEG, Ashley CC, Castell LM, Somers V, Osborn C, Ledingham JGG, Conway J (1989) Correlation of blood pressure in normotensive and hypertensive individuals with platelet but not lymphocyte intracellular free calcium concentrations. Clin Sci 76:631–635

    Google Scholar 

  30. Schiffl H, Weidmann P, Beretta-Piccoli C, Cottier C, Sciler AJ, Ziegler WH (1982) Antihypertensive mechanism of the diuretic muzolimine in mild renal failure. Eur J Clin Pharmacol 23:215–220

    Google Scholar 

  31. Schiffl H (1989) Platelet cytosolic free calcium concentration in hypertension associated with early-stage kidney disease. Klin Wochenschr 67:676–681

    Google Scholar 

  32. Schiffl H (1990) Correlation of blood pressure in end-stage renal disease with platelet cytosolic free-calcium concentration. Klin Wochenschr 68:718–722

    Google Scholar 

  33. Tsien RY, Pozzan T, Rink TJ (1982) Calcium homeostasis in intact lymphocytes, cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol 94:325–334

    Google Scholar 

  34. Ueno H, Mikawa M, Takata M, Aasnoi H, Lida H, Sasayama S, Kagamimori S (1988) Does cytosolic free calcium concentration in platelets reflect tone and structural changes of resistance vessels? J Hypertens 6 [Suppl 4]:S255-S257

    Google Scholar 

  35. Zemel MB, Zemel PC, Berry S, Norman G, Kowalczyk C, Sokol RJ, Standly PR, Walsh MF, Sowers JR (1990) Altered platelet calcium metabolism as an early predictor of increased peripheral vascular resistance and preeclampsia in urban black women. N Engl J Med 323:434–438

    Google Scholar 

  36. Zidek W, Sachinidis A, Spieker C, Storkebaum W (1988) Effect of plasma from hypertensive subjects on Ca2+ transport in permeabilized human neutrohils. Clin Sci 74:53–56

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schiffl, H. Modulation of platelet Ca2+ homeostasis by hypertensive plasma factor(s) derived from patients with early-stage renal disease. Klin Wochenschr 69, 917–923 (1991). https://doi.org/10.1007/BF01798539

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01798539

Key words

Navigation