Journal of Molecular Evolution

, Volume 3, Issue 1, pp 63–77 | Cite as

The use of ribonuclease U2 in RNA sequence determination

Some Corrections in the Catalog of Oligomers Produced by Ribonuclease T, Digestion ofEscherichia coli 16S Ribosomal RNA
  • T. Uchida
  • L. Bonen
  • H. W. Schaup
  • B. J. Lewis
  • L. Zablen
  • C. Woese
Article

Abstract

The catalog of oligomers produced by ribonuclease T1 digestion ofEscherichi coli 16S ribosomal RNA has been determined by a new method that involves the use of ribonuclease U2 fromUstilago sphaerogena. The sequences for the larger T1 oligomers (8 or more bases) determined in this way differ in more than 50 % of the cases from those reported previously (determined by other methods).

Key words

16S Ribosomal RNA Oligonucleotide Fingerprint Ribonuclease U2 Escherichia coli 

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • T. Uchida
    • 1
  • L. Bonen
    • 1
  • H. W. Schaup
    • 1
  • B. J. Lewis
    • 1
  • L. Zablen
    • 1
  • C. Woese
    • 1
  1. 1.Department of MicrobiologyUniversity of IllinoisUrbanaUSA

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