Summary
We have studied the metal-ion catalysis of a number of reactions of the isomers of ImpGpG on a poly(C) template. In the absence of a catalytic metal ion, oligomers at least up to (pG)20 are obtained from the ImpGpG isomers in a l-methylimidazole buffer. The Pb2+ ion improves the yield of longer oligomers and changes substantially the distribution of linkage isomers. The Pb2+ ion greatly improves the yield of longer oligomers obtained from G and ImpGpG on a poly(C) template.
The self-condensation of ImpGpG in a 2, 6-lutidine buffer is much less efficient than in a l-methylimidazole buffer. The Zn2+ greatly increases the yield of products from the [3′-5′]-linked dimer, but fails to catalyze the formation of long oligomers from the [2′-5′]-linked dimer. The bonds formed in the Zn2+-catalyzed selfcondensation of ImpG3pG on poly(C) are mainly [3′-5′]-linked.
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Abbreviations
- Im:
-
imidazole
- MeIm:
-
l-methylimidazole
- EDTA:
-
ethylenediamine tetraacetic acid
- A:
-
adenosine
- pA:
-
adenosine 5′-phosphate
- G:
-
guanosine
- Gp:
-
guanosine 2′(3′)-phosphate
- pG:
-
guanosine 5′-phosphate
- ImpG:
-
guanosine 5′-phosphorimidazolide
- ImpGpG:
-
5′-imidazolide of a diguanylate pGpG
- (Gp)n (n= 2, 3⋯):
-
oligomers of Gp
- (pG)n (n = 2, 3⋯):
-
oligomers of pG
- G(pG)n :
-
oligoguanylates terminated by a free 5′-OH group.
- poly(C):
-
polycytidylic acid
- poly(U):
-
polyuridylic acid
- poly(A):
-
polyadenylic acid
References
Bridson PK, Orgel LE (1980) J Mol Biol 144:567–577
Lohrmann R, Orgel LE (1979a) J Mol Evol 12:237–257
Lohrmann R, Orgel LE (1979b) J Mol Evol 14:243–250
Lohrmann R, Orgel LE (1980) J Mol Biol 142:555–567
Lohrmann R, Bridson PK, Orgel LE (1980) Science 208:1464–1465
Ninio J, Orgel LE (1978) J Mol Evol 12:91–99
Orgel LE, Lohrmann R (1974) Accounts of Chem Res 7:368–377
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The number given as superscript between a nucleoside and a phosphate indicates the type of internucleotide linkage, e.g. pG2pG, 5′-phospho-guanylyl [2′→5′]-guanosine. A star above the symbol for a nucleoside indicates the position of the radioactive label e. g. G3pG3pG [2-14C]-guanylyl-[3′→5′]-guanylyl-[3′→5′]-guanosine;
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Lohrmann, R., Bridson, P.K. & Orgel, L.E. Condensation of activated diguanylates on a poly(C) template. J Mol Evol 17, 303–306 (1981). https://doi.org/10.1007/BF01795752
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DOI: https://doi.org/10.1007/BF01795752