Inflammation Research

, Volume 44, Issue 2, pp 70–73 | Cite as

Bradykinin-induced vasodilation is changed to a vasoconstrictor response in vessels of aged normotensive and hypertensive rats

  • Laura Mantelli
  • Sandra Amerini
  • Fabrizio Ledda
Article

Abstract

The vascular response to bradykinin was investigated in mesenteric vascular bed preparations preconstricted with methoxamine, obtained from 2- and 18-month old normotensive (WKY) and spontaneously hypertensive (SHR) rats. In preparations from young normotensive rats bradykinin (1 nm-10 µM) produced an endothelium-dependent vasorelaxant effect which was greatly reduced by the B2 receptor antagonist Ac-D-Arg[Hyp3,D-Phe7,Leu8]-bradykinin (1 µM), and was unaffected by the B1 receptor antagonist des-Arg9,[Leu8]-bradykinin (1 µM). The degree of vasodilation was similar in preparations from age-matched SHR rats. In vessels obtained from old animals bradykinin induced an endothelium-independent vasoconstrictor response; this effect was more pronounced in preparations from SHR than in those from WKY rats. The vasoconstriction was unaffected by both B1 and B2 receptor antagonists, and was abolished by 3 µM indomethacin. We conclude that the vasorelaxant effect of bradykinin in vessels of young animals is due to stimulation of B2 receptors. This vasodilating response can be converted by aging to a vasoconstriction and is probably due to the release of a prostanoid product; moreover it is more pronounced in spontaneously hypertensive animals.

Key words

Bradykinin Spontaneously hypertensive rats (SHR) Mesenteric vascular bed Aging 

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Copyright information

© Birkhäuser Verlag 1995

Authors and Affiliations

  • Laura Mantelli
    • 1
  • Sandra Amerini
    • 1
  • Fabrizio Ledda
    • 1
  1. 1.Department of PharmacologyUniversity of FlorenceFirenzeItaly

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