Skip to main content
Log in

The use of alternative substrates in steady-state enzyme kinetics: A re-examination of the criteria for distinguishing between sequential bi bi mechanisms

  • General and Review Articles
  • a. general articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

A new set of criteria based on the use of alternative substrates, is proposed for distinguishing between sequential bi bi enzyme mechanisms. Some of the criteria previously advocated are shown to be invalid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Fromm, H. J., Biochim. Biophys. Acta. 81, 413–417, 1964.

    PubMed  Google Scholar 

  2. Zewe, V., Fromm, H. J. and Fabiano, R., J. Biol. Chem. 239, 1625–1634, 1964.

    PubMed  Google Scholar 

  3. Rudolph, F. B. and Fromm, H. J., Biochemistry 9, 4660–4665, 1970.

    PubMed  Google Scholar 

  4. Cleland, W. W., Biochim. Biophys. Acta. 67, 104–137, 1963.

    PubMed  Google Scholar 

  5. Wong, J. T.-F. and Hanes, C. S., Can. J. Biochem. Physiol. 40, 763–804, 1962.

    PubMed  Google Scholar 

  6. Cleland. W. W., Biochim. Biophys. Acta. 67, 173–187, 1963.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Darvey, I.G. The use of alternative substrates in steady-state enzyme kinetics: A re-examination of the criteria for distinguishing between sequential bi bi mechanisms. Mol Cell Biochem 11, 3–8 (1976). https://doi.org/10.1007/BF01792829

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01792829

Keywords

Navigation