Summary
A new set of criteria based on the use of alternative substrates, is proposed for distinguishing between sequential bi bi enzyme mechanisms. Some of the criteria previously advocated are shown to be invalid.
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Darvey, I.G. The use of alternative substrates in steady-state enzyme kinetics: A re-examination of the criteria for distinguishing between sequential bi bi mechanisms. Mol Cell Biochem 11, 3–8 (1976). https://doi.org/10.1007/BF01792829
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DOI: https://doi.org/10.1007/BF01792829