Cancer Immunology, Immunotherapy

, Volume 31, Issue 4, pp 197–201 | Cite as

Effects of methotrexate-carcinoembryonic-antigen-antibody immunoconjugates on GW-39 human tumors in nude mice

  • Lisa B. Shih
  • David M. Goldenberg
Original articles

Summary

Methotrexate (MTX) was conjugated to an anti-carcinoembryonic antigen monoclonal antibody (NP2) by using amino-dextran as an intermediate carrier. The drug was chemically linked to amino-dextran (averageMr = 40000), and the resulting MTX-dextran was then site-specifically attached to the carbohydrate moiety of the antibody. Athymic nude mice that carried human colonic GW-39 tumors (s. c.) were treated with the immunoconjugate. In this study, the specific conjugate caused a greater inhibition of the tumor growth than either free MTX or its conjugate with dextran and an irrelevant antibody. The intermediate MTX-dextran and the unlinked mixture of MTX-dextran with NP2 were both relatively ineffective in inhibiting tumor growth. The greatly reduced host toxicity permitted the use of the MTX-dextran-NP2 in a high-dose therapy of this tumor system.

Keywords

Tumor Growth Methotrexate Dextran Nude Mouse Human Tumor 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Lisa B. Shih
    • 1
  • David M. Goldenberg
    • 1
  1. 1.Center for Molecular Medicine and ImmunologyUniversity of Medicine and Dentistry of New JerseyNewarkUSA

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