Summary
Proteins from ribosomes of different tissues and animals were characterized by polyacrylamide disc electrophoresis. The proteins from ribosomes of different tissues from the same animal are qualitatively similar. The results of the experiments with ribosomes from the livers of different species of animals exhibit clear differences, in the electrophoretic patterns of the proteins.
Similar content being viewed by others
References
Bielka, H., andH. Welfle: On ribosomal proteins, characterized by polyacrylamide gel-and immuno-electrophoresis. Internat. Symposium on Biochemistry of Ribosomes and Messenger-RNA. Reinhardsbrunn (Thüringen), 23 May 1967.
Cox, E. C., and J. G.Flaks: Strepotmycin action and the ribosome. Fed. Proc.23, 220 (1964).
Curry, J. B., and R. T.Hersh: Molecular weight of the protein from bovine liver ribosomes. Biochem. Biophys. Res. Commun.6, 415 (1962).
Furano, A. V.: Chromatography ofEscherichia coli ribosomes on diethylaminoethyl cellulose. J. biol Chem.241, 2237 (1966).
Gesteland, R. F., and T.Staehelin: Electrophoretic analysis of proteins from normal and caesium chloride-treatedE. coli ribosomes. J. molec. Biol.24, 149 (1967).
LeBoy, P. S., E. C.Cox, and J. G.Flaks: The chromosomal site specifying a ribosomal protein inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)52, 1367 (1964).
Low, R. B., and I. G.Wool: Mammalian ribosomal protein: Analysis by electrophoresis on polyacrylamide gel. Science155, 330 (1967).
Matson, Ch. F.: Polyacrylamide gel electrophoresis. A simple system using gel columns. Analyt. Biochem.13, 294 (1965).
Ornstein, R., and B. J.Davis: Disc electrophoresis — I. Background and theory. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci.121, 321, 404 (1964).
Tashiro, Y., and Ph.Siekevitz: Ultracentrifugal studies on the dissociation of hepatic ribosomes. J. molec. Biol.11, 149 (1965).
Traub, P., M.Nomura, and L.Tu: Physical and functional heterogeneity of ribosomal proteins. J. molec. Biol.19, 215 (1966).
Traut, R. R.: Acrylamide gel electrophoresis of radioactive ribosomal protein. J. molec. Biol.21, 571 (1966).
Waller, J. P.: Fractionation of the ribosomal protein fromEscherichia coli. J. molec. Biol.10, 319 (1964).
—, and J. I.Harris: Studies on the composition, of the protein fromEscherichia coli ribosomes. Proc. nat. Acad. Sci. (Wash.)47, 18 (1961).
Welfle, H, u. H.Bielka: Charakterisierung von Proteinen aus Leber- und Hepatom-Ribosomen durch Polyacrylamid-Gelelektrophorese. Z. Naturforsch.23b, 690 (1968).
Author information
Authors and Affiliations
Additional information
Communicated byH.G. Wittmann
Rights and permissions
About this article
Cite this article
Bielka, H., Welfle, H. Characterization of ribosomal proteins from different tissues and species of animals by electrophoresis on polyacrylamide gel. Molec. Gen. Genet. 102, 128–131 (1968). https://doi.org/10.1007/BF01789139
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01789139