Molecular and General Genetics MGG

, Volume 102, Issue 2, pp 95–101 | Cite as

N-formylmethionine as a N-terminal group ofE. coli ribosomal protein

  • Peter Hauschild-Rogat


Enzymatic digestion of35S ribosomal protein with pronase yielded 0.14 molar % of the amino acids as N-formylmethionine. Analysis showed that approximately two polypeptide chains in the protein of the 30 S subparticle and approximately nine in the 50 S subparticle start with N-formylmethionine.


Polypeptide Enzymatic Digestion Ribosomal Protein Polypeptide Chain 


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  1. Adams, J. M., and M. R.Capecchi: N-formylmethionyl-sRNA as the initiator of protein synthesis. Proc. nat. Acad. Sci. (Wash.)55, 147 (1966).Google Scholar
  2. Allfrey, V. G., R.Faulkner, and A. E.Mirsky: Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc. nat. Acad. Sci. (Wash.)51, 786 (1964).Google Scholar
  3. Bretscher, M. S., and K. A.Marker: Polypeptidyl-s-ribonucleic acid and amino-acyl-s-ribonucleic acid binding sites on ribosomes. Nature (Lond.)211, 380 (1966).Google Scholar
  4. Cox, E. C., and J. G.Flaks: Streptomycin action and the ribosome. Fed. Proc.23, 220 (1964).Google Scholar
  5. Dekker, C. A., D.Stone, and J. S.Fruton: A peptide from a marine alga. J. biol. Chem.181, 719 (1949).PubMedGoogle Scholar
  6. Feigl, F.: Spot tests in organic analysis, p. 368, 6. ed. (1960).Google Scholar
  7. Kaltschmidt, E., M.Dzionara, D.Donner, and H. G.Wittmann: Ribosomal proteins I. Isolation, amino acid composition, molecular weights and peptide mapping of proteins fromE. coli ribosomes. Molec. Gen. Genetics100, 304 (1967).Google Scholar
  8. Kreil, G., and G.Kreil-Kiss: The isolation of N-formylglycine from a polypeptide present in bee venom. Biochem. biophys. Res. Commun.2, 275 (1967).Google Scholar
  9. Levy, A. L.: A paper chromatographic method for the quantitative estimation of amino acids. Nature (Lond.)174, 126 (1954).Google Scholar
  10. Möller, W., and A.Chrambach: Physical heterogenity of the ribosomal proteins fromE. coli. J. molec. Biol.23, 377 (1967).PubMedGoogle Scholar
  11. Nomoto, M., Y.Narahashi, and M.Murakami: A proteolytic enzyme ofStreptomyces griseus VII. Substrate specifity ofStreptomyces griseus protease. J. Biochem.48, 906 (1960).Google Scholar
  12. Osteux, R. O., J.Guillaume et Y.Laturaze: Séparation des acides aliphatiques volatils par chromatographie sur papier. J. chromatog.1, 70 (1958).Google Scholar
  13. Press, E. M., P. J.Piggot, and R. R.Porter: Terminal sequences of a human Ig G heavy chain. Biochem. J.99, 356 (1966).PubMedGoogle Scholar
  14. Sinsheimer, R. L., B.Starman, C.Nagler, and S.Guthrie: The process of infection with bacteriophage ϕX174. I. Evidence for a “replicative form”. J. molec. Biol.4, 142 (1962).PubMedGoogle Scholar
  15. Spitnik-Elson, P.: The solubilisation of ribosomal protein fromEscherichia coli. Biochim. biophys. Acta (Amst.)55, 741 (1962).Google Scholar
  16. Traut, R. R., P. B.Moore, H.Delius, H.Noller, and A.Tissières: Ribosomal proteins ofEscherichia coli. I. Demonstration of different primary structures. Proc. nat. Acad. Sci. (Wash.)57, 1294 (1967).Google Scholar
  17. Tzagoloff, H., and D.Pratt: The initial steps in infection with ColiphageM13. Virology24, 372 (1964).Google Scholar
  18. Waller, J. P.: Fractionation of the ribosomal proteins fromEscherichia coli. J. molec. Biol.10, 319 (1964).PubMedGoogle Scholar
  19. —, and J. I.Harris: Studies on the composition of the protein fromE. coli ribosomes. Proc. nat. Acad. Sci. (Wash.)47, 18 (1961).Google Scholar
  20. Webster, R. E., D. L.Engelhardt, and M. D.Zinder: In vitro protein synthesis: chain initiation. Proc. nat. Acad. Sci. (Wash.)55, 155 (1966).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Peter Hauschild-Rogat
    • 1
    • 2
  1. 1.Max-Planck-Institut für VirusforschungTubingenGermany
  2. 2.Institut für Biologie (Genetik) der UniversitätTübingen

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