Summary
Transfer of a non-Mendelian neamine-dependent (nd) mutant to an antibioticfree medium results in neamine-sensitive and neamine-resistent revertants. These reversions are caused by extranuclear mutations.
The neamine-sensitive revertants are no more able to split offnd-cells after back-donation to neamine containing medium. Therefore they are different from the streptomycin-sensitive revertants of a streptomycin-dependent (sd) mutant. These mutants were capable ofsd-segregation though their potence ofsd-segregation diminished on antibiotic-free medium with increasing time of cultivation.
The different behaviour can be explained by the fact that manysd-genes are present which have to be appointed to the mitochondria. On the other side, thend-gene exists only in few copies and is located therefore in the chloroplast.
Several experiments with differing methods are discussed to localize the extranuclear genes.
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Behn, W., Arnold, C.G. Zur Lokalisation eines nichtmendelnden Gens vonChlamydomonas reinhardii . Molec. Gen. Genet. 114, 266–272 (1972). https://doi.org/10.1007/BF01788896
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DOI: https://doi.org/10.1007/BF01788896