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In vivo activation of NK cells induces inhibition of lung colonization of H-2 positive and H-2 negative fibrosarcoma tumor clones

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Abstract

The role of different tilorone analogs in the abrogation of the metastatic spread of H-2 positive and H-2 negative tumor clones was studied. Pre-treatment of BALB/c mice with RMI 10,874DA compound completely abolished lung colonization of an H-2 negative (GR9.B9) MCA-induced fibrosarcoma clone in an experimental metastasis assay. This effect was also evident when clones were treated with other tilorone analogs (R11,567DA or R11,513DA). Other H-2 positive and H-2 negative chemically induced fibrosarcoma clones were also tested. The effect was not due to direct toxicity of the tilorone analog on tumor cells, but instead was dependent on NK cells; this was suggested by the finding that treatment of mice with anti-asialo GM1 abrogated the effect of the tilorone analog (RMI 10,874DA compound). Interestingly, the inhibition of lung colonization after intravenous injection was again observed regardless of the H-2 phenotype of the tumor clones, and H-2+ and H-2− clones were similarly inhibited.In vitro assays of NK sensitivity of tumor clones showed that lysis varied depending on the H-2 phenotype of tumor clones, indicating an absence of correlation betweenin vivo andin vitro results.

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Authors and Affiliations

  1. Department of Clinical Analysis and Immunology, Hospital Virgen de las Nieves, Universidad de Granada, E-18014, Granada, Spain

    Ignacio Algarra, Matías Pérez, Jose Juan Gaforio, Fernando Gasca & Federico Garrido

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  1. Ignacio Algarra
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  2. Matías Pérez
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  3. Jose Juan Gaforio
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  4. Fernando Gasca
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  5. Federico Garrido
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Algarra, I., Pérez, M., Gaforio, J.J. et al. In vivo activation of NK cells induces inhibition of lung colonization of H-2 positive and H-2 negative fibrosarcoma tumor clones. Clin Exp Metast 12, 31–36 (1994). https://doi.org/10.1007/BF01784331

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  • DOI: https://doi.org/10.1007/BF01784331

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