Inflammation Research

, Volume 44, Supplement 2, pp S131–S132 | Cite as

Metalloprotease inhibitors halt collagen breakdown in IL-1 induced bovine nasal cartilage cultures

  • S. Spirito
  • J. Doughty
  • E. O'Byrne
  • V. Ganu
  • R. L. Goldberg
I. Cartilage degradation and osteoarthritis

Keywords

Collagen Nasal Cartilage Collagen Breakdown Metalloprotease Inhibitor Bovine Nasal Cartilage 

References

  1. [1]
    Stephenson ML, Goldring MB, Birkhead JR, Krane SM, Rahmsdorf HJ, Angel P. Stimulation of procollagenase synthesis parallels increases in cellular procollagenase mRNA in human articular chondrocytes exposed to recombinant interleukin-1β or phorbol ester. Biochem Biophys Res Comm 1987;144:583–90.PubMedGoogle Scholar
  2. [2]
    Ganu VS, Hu S-I, Melton R, Winter C, Goldberg VM, Haqqi TM, Malemud CJ. Biochemical and molecular characterization of stromelysin synthesized by human osteoarthritic chondrocytes stimulated with recombinant human interleukin-1. Clin Exp Rheumatol 1994;12.Google Scholar
  3. [3]
    Tyler JA. IGF-1 can decrease degradation and promote synthesis of proteoglycan in cartilage exposed to cytokines. Biochem J 1989;260:543–8.Google Scholar
  4. [4]
    Caputo CB, Sygowski LA, Wolanin DJ, Patton SP, Caccese RG, Shaw A, et al. Effect of synthetic metalloprotease inhibitors on cartilage autolysis in vitro. J Pharmacol Exp Ther 1987;240:460–5.PubMedGoogle Scholar
  5. [5]
    Nixon JS, Bottomley KK, Broadhurst MJ, Brown PA, Johnson WH, Lawton G, et al. Potent collagenase inhibitors prevent interleukin-1 induced cartilage degradation in vitro. Int J Tiss React Expt Clin Aspects 1991;13:237–41.Google Scholar
  6. [6]
    Goldberg RL, Kolibas LM. An improved method for determining proteoglycans synthesized by chondrocytes in culture. Conn Tiss Res 1990;24:265–75.Google Scholar
  7. [7]
    Woessner JF. The determination of hydroxyproline in tissue and protein samples containing small portions of this imino acid. Arch Biochem Biophys 1961;93:440–7.PubMedGoogle Scholar
  8. [8]
    Sandy JD, Neame PJ, Boynton RE, Flannery CR. Catabolism of aggrecan in cartilage explants. Identification of a major cleavage site within the interglobular domain. J Biol Chem 1991;266:8683–5.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1995

Authors and Affiliations

  • S. Spirito
    • 1
  • J. Doughty
    • 1
  • E. O'Byrne
    • 1
  • V. Ganu
    • 1
  • R. L. Goldberg
    • 1
  1. 1.Pharmaceuticals Div., Research DepartmentCiba-Geigy Corp.SummitUSA

Personalised recommendations