Summary
Expression of a foreign protein molecule on theE. coli bacterial surface has been achieved through hybrid plasmid construction of fusion proteins using outer membrane proteinompA as a carrier system. Influenza virus hemagglutinin fusion proteins of this character have been shown to become integrated into the bacterial outer membrane and to expose their hemagglutinin moiety at the exterior surface in a conformation which is at least similar to the authentic viral antigen structure.
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Abbreviations
- bp:
-
Base pairs (DNA)
- kD:
-
kiloDaltons (protein)
- HA:
-
Hemagglutinin
- IPTG:
-
Isopropylthiogalactoside
- PBS:
-
Phosphate buffered saline
- PVDF:
-
Polyvinyldifluoride
References
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Dedicated to Professor Dr. N. Zöllner on the occasion of his 65th birthday
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Pistor, S., Hobom, G. Expression of viral hemagglutinin on the surface ofE. coli . Klin Wochenschr 66, 110–116 (1988). https://doi.org/10.1007/BF01774224
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DOI: https://doi.org/10.1007/BF01774224