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Journal of Muscle Research & Cell Motility

, Volume 9, Issue 5, pp 370–383 | Cite as

Fluorescent phallotoxins as probes for filamentous actin

  • Heinz Faulstich
  • S. Zobeley
  • G. Rinnerthaler
  • J. V. Small
Review

Conclusions

Phallotoxins have been conjugated to four different fluorescent labels: fluorescein, tetramethylrhodamine, nitrobenzoxadiazole and coumarine. Although endowed with different substrate affinities, they are all specific for polymeric actin.

Useful advantage can and has been taken of the special characteristics of the fluorescent phallotoxin derivatives. Among them:
  1. 1.

    Their specific labelling of F-actin, native or chemically fixed, in a simple one-step procedure, making them useful probes for single and multiple fluorescent labelling of cytoskeletal and other preparations.

     
  2. 2.

    Their stabilization of F-actin against depolymerization and benign effects on actomyosin-based movement, important factors inin vitro motility systems. The stabilizing effect of phalloidin leads also to improved structural preservation in immunofluorescence preparations.

     
  3. 3.

    Their small size, allowing ready penetration into cytoskeletal networks, in fixed and living cells.

     
  4. 4.

    Their long-term stability, dry or in organic solvents.

     

From their various useful properties we can expect fluorescent phallotoxins to find further and new applications in studies of actin-based phenomena.

Keywords

Organic Solvent Fluorescein Coumarine Phalloidin Fluorescent Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd 1988

Authors and Affiliations

  • Heinz Faulstich
    • 1
  • S. Zobeley
    • 1
  • G. Rinnerthaler
    • 2
  • J. V. Small
    • 2
  1. 1.Abteilung PhysiologieMax-Planck-Institut für Medizinische ForschungHeidelbergF.R.G.
  2. 2.Institute of Molecular Biology of the Austrian Academy of SciencesSalzburgAustria

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