Summary
The yeastSaccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.
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Aspenström, P., Lassing, I. & Karlsson, R. Production, isolation and characterization of human profilin fromSaccharomyces cerevisiae . J Muscle Res Cell Motil 12, 201–207 (1991). https://doi.org/10.1007/BF01774039
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DOI: https://doi.org/10.1007/BF01774039