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Journal of Muscle Research & Cell Motility

, Volume 12, Issue 2, pp 201–207 | Cite as

Production, isolation and characterization of human profilin fromSaccharomyces cerevisiae

  • Pontus Aspenström
  • Ingrid Lassing
  • Roger Karlsson
Papers

Summary

The yeastSaccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.

Keywords

Recombinant Protein Affinity Chromatography Exchange Chromatography Human Profilin Profilin cDNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd 1991

Authors and Affiliations

  • Pontus Aspenström
    • 1
  • Ingrid Lassing
    • 2
  • Roger Karlsson
    • 1
  1. 1.Department of Developmental BiologyUppsala University, Biomedical CenterUppsalaSweden
  2. 2.Department of Zoological Cellbiology, Arrhenius Laboratories E5Stockholm UniversityStockholmSweden

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