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Protective effect of ascorbic acid against lipid peroxidation and oxidative damage in cardiac microsomes

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Abstract

One of the current theories of cardiovascular disease is that it may begin with oxygen radical-induced damages. Extensive studies have been made in different laboratories to elucidate the mechanism of oxidative damages in the presence of added iron salts. However, thosein vitro studies are unlikely to be relevant to thein vivo situation, where in the normal physiological condition most of the iron remains bound with proteins. In the present study we have demonstrated that anin vitro system containing desferrioxamine, a strong iron chelator, superoxide generated by the action of xanthine oxidase on acetaldehyde initiates lipid peroxidation and protein changes in the guinea pig cardiac microsomes. We have further demonstrated that superoxide-initiated lipid peroxidation and protein changes are completely prevented by ascorbic acid. SOD also prevents but catalase, α-tocopherol, glutathione, uric acid, thiourea, mannitol and histidine are without effect. When NADPH is used instead of generated superoxide, the lipid peroxidation and protein changes are exclusively inhibited by ascorbic acid. SOD, catalase and other antioxidants are ineffective. The results obtained with guinea pigs may be extrapolated to humans, because like guinea pigs humans are also incapable of synthesizing ascorbic acid.

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Authors and Affiliations

  1. Department of Biochemistry, University College of Science, 35 Ballygunge Circular Road, 700 019, Calcutta, India

    Mau Mukhopadhyay, Chinmay K. Mukhopadhyay & Indu B. Chatterjee

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  1. Mau Mukhopadhyay
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  2. Chinmay K. Mukhopadhyay
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  3. Indu B. Chatterjee
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Mukhopadhyay, M., Mukhopadhyay, C.K. & Chatterjee, I.B. Protective effect of ascorbic acid against lipid peroxidation and oxidative damage in cardiac microsomes. Mol Cell Biochem 126, 69–75 (1993). https://doi.org/10.1007/BF01772209

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