Clinical & Experimental Metastasis

, Volume 6, Issue 5, pp 363–375 | Cite as

Characterization of a connective tissue degrading metalloproteinase from human small cell lung cancer cells

  • Stanley Zucker
  • Taina Turpeeniemi-Hujanen
  • Janine M. Wieman
  • Rita M. Lysik


In this report. we describe the isolation and characterization of a neutral metalloproteinase, from human small cell lung cancer cells, which degrades a wide range of connective tissue proteins. Treatment of tumor cytosol by ammonium sulphate precipitation followed by zinc chelated column chromatography, anion exchange chromatography, and gel filtration chromatography yielded a single enzymatically active protein, which on SDS-PAGE appeared as a diffuse band of 65 000–70 000 daltons. The tumor metalloproteinase, which was inhibited by metal chelators and serum, was able to digest gelatin, type I collagen, type IV collagen, laminin, and fibronectin. We propose that the capacity of this proteinase to degrade both components of blood vessel basement membranes and other connective tissue matrices facilitates the dissemination of human lung cancer cells during the multistep process of metastasis.


Gelatin Laminin Ammonium Sulphate Metal Chelator Human Lung Cancer 
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Copyright information

© Taylor & Francis Ltd 1988

Authors and Affiliations

  • Stanley Zucker
    • 1
    • 2
    • 3
    • 5
  • Taina Turpeeniemi-Hujanen
    • 4
    • 3
    • 5
  • Janine M. Wieman
    • 2
    • 3
    • 5
  • Rita M. Lysik
    • 2
    • 3
    • 5
  1. 1.Department of MedicineVeterans Administration Medical CenterNorthportUSA
  2. 2.Department of ResearchVeterans Administration Medical CenterNorthportUSA
  3. 3.School of MedicineState University of New York at Stony BrookStony BrookUSA
  4. 4.Collagen Research UnitFinland
  5. 5.Department of Medical BiochemistryUniversity of OuluOulu 22Finland

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