Abstract
The purpose of these studies was to determine whether the high macrophage content (>50 per cent) of the90Sr-induced osteogenic sarcoma J (Os-J) of recent origin correlated with its immunogenicity or low metastatic potential. Cloning experiments demonstrated that the Os-J tumor is heterogeneous with regard to the production of experimental pulmonary metastases. Immunization-challenge studies in syngeneic mice and comparisons of tumor growth in normal or nude mice established that the slow growing Os-J tumor is poorly immunogenic.In vitro studies demonstrated that the Os-J tumor is highly susceptible to macrophages-mediated lysis. This may explain the slow growth of the tumor in normal recipients with an intact mononuclear phagocyte system, as compared with the more rapid emergence of tumors in macrophage-suppressed mice. However, spontaneous metastases of the Os-J tumor were not observed either in normal or macrophage-suppressed mice. Although a high macrophage infiltration of neoplasms could slow tumor growth, this was not associated with the immunogenicity of the neoplasm and did not appear to limit the spontaneous metastasis of this essentially benign neoplasm.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Acero, R., Polentarutti, N., Bottazzi, B., Alberti, S., Ricci, M. R., Bizzi, A., andMantovani, A., 1984, Effect of hydrocortisone on the macrophage content, growth and metastasis of transplanted murine tumors.International Journal of Cancer,33, 95–105.
Argyris, B. F., andReif, A. E., 1981, Lack of suppressor cell activity in the spleens of mice with radiation-induced osteogenic sarcomas.Cancer Research,41, 839–844.
Eccles, S. A., andAlexander, P., 1974, Macrophage content of tumors in relationship to metastatic spread.Nature,250, 667–669.
Evans, R., andEidlen, D. M., 1981, Macrophage accumulation in transplantable tumors is not dependent on host immune responsiveness or presence of tumor-associated rejection antigens.Journal of the Reticuloendothelial Society,30, 425–437.
Evans, R., andLawler, E. M., 1980, Macrophage content and immunogenicity of C57BL/6J and Balb/cByJ methylcholanthrene-induced sarcomas.International Journal of Cancer,26, 831–835.
Fidler, I. J., 1977, Depression of macrophages in mice drinking hyperchlorinated water.Nature,270, 735–736.
Fidler, I. J., Gersten, D. M., andHart, I. R., 1978, The biology of cancer invasion and metastasis.Advances in Cancer Research,28, 149–250.
Gauci, G. L., andAlexander, P., 1975, The macrophage content of some human tumors.Cancer Letters,1, 29–34.
Hart, I. R., Talmadge, J. E., andFidler, I. J., 1981, Metastatic behavior of a murine reticulum cell sarcoma exhibiting organ-specific growth.Cancer Research,41, 1281–1287.
Herberman, R. B., Djeu, J. R., Kay, H. D., Ortaldo, J. R., Riccardi, D., Bonnard, G. D., Holden, H. T., Fagnani, R., Santoni, A., andPucetti, P., 1979, Natural killer cells: Characteristics and regulation of activity.Immunology Reviews,44, 43–63.
Keller, R., 1976, Promotion of tumor growthin vivo by anti-macrophage agents.Journal of the National Cancer Institute,57, 1355–1361.
Key, M. E., 1983, Macrophages in cancer metastases and their relevance to metastatic growth.Cancer Metastasis Reviews,2, 75–88.
Kerbel, R. S., Twiddy, R. R., 1980, Host cell analysis of a rapidly metastasizing mouse tumor and derived low metastatic variant lines.Contemporary Topics in Immunology, edited by I. P. Witz and M. G. Hanna (New York: Plenum Press), pp. 239–254.
Kripke, M. L., Gruys, E., andFidler, I. J., 1978, Metastatic heterogeneity of cells from an ultraviolet light-induced murine fibrosarcoma of recent origin.Cancer Research,38, 2962–2967.
Moore, M., andWilliams, D. E., 1972, Studies on the antigenicity of radiation-induced murine osteosarcomata.British Journal of Cancer,26, 90–98.
Moore, M., andWilliams, D. E., 1973, Tumor-specific antigenicity of osteosarcomas induced in rodents by bone-seeking radionuclides.Radionuclides Carcinogenesis, edited by C. L. Sanders, R. H. Bush, J. E. Ballow, and D. D. Mahlum (Springfield, Illinois: U.S. Atomic Energy Commission) U.S. Atomic Energy Commission Symposium Series, Vol. 29, pp. 289–306.
Nash, J. R. G., Price, J. E., andTarin, D., 1981, Macrophage content and colonyforming potential in mouse mammary carcinomas.British Journal of Cancer,39, 478–485.
Nicolson, G. L., andCustead, S. E., 1982, Tumor metastasis is not due to adaptation of cells to a new organ environment.Science,215, 176–178.
Pross, H. F., andKerbel, R. S., 1976, An assessment of intratumor phagocytic surface marker-bearing cells in a series of autochthonous and early passaged chemically induced murine sarcomas.Journal of the National Cancer Institute,57, 1157–1161.
Poste, G., Kirsh, R., Fogler, W., andFidler, I. J., 1979, Activation of tumoricidal properties in mouse macrophages by lymphokines encapsulated in liposomes.Cancer Research,39, 881–885.
Reif, A. E., 1982, Antigenicity of tumors: A comprehensive system of measurement.Methods in Cancer Research, edited by H. Busch and L. C. Yeoman (New York: Academic Press), Vol. XX, pp. 4–84.
Talmadge, J. E., Key, M., andFidler, I. J., 1981, Macrophage content of metastatic and nonmetastatic rodent neoplasms.Journal of Immunology,126, 2245–2248.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Talmadge, J.E., Uithoven, K.A. & Reif, A.E. Relationship of macrophage content, immunogenicity, and metastatic potential of a murine osteosarcoma of recent origin. Clin Exp Metast 3, 61–72 (1985). https://doi.org/10.1007/BF01758954
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01758954