Abstract
Cell debris is inevitably produced by the mechanical and enzymatic procedures used to dissociate solid tumors. We investigated the influence of such debris on the i.v. and s.c. transplantability of B16 melanoma cells. Cell suspensions were centrifuged on a Percoli cushion to remove the debris, and the purified preparations were compared to untreated control suspensions. The i.v. injection of a massive dose (106 cells) of unpurified cells killed the animals within a few minutes, while the same amount of purified cells left the animals unaffected. When 5×105 cells were given, animals receiving purified cell suspensions had more extrapulmonary tumors at their time of death than those given unpurified cells. However, with a lower cell dose, 2×104 cells, there were no such differences in tumor incidence or distribution.
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Bosslet, K., Ruffmann, R., Altevogt, P., andSchirrmacher, V., 1981, A rapid method for the isolation of metastasizing tumour cells from internal organs with the help of isopycnic density-gradient centrifugation in Percoll.British Journal of Cancer,44, 356–362.
Erkell, L. J., Ryd, W., andHagmar, B., 1984, The tumor cell release machine.Invasion and Metastasis,4, 126–133.
Fidler, I. J., 1978, General considerations for studies of experimental cancer metastasis.Methods in Cancer Research,15, 399–439.
Grdina, D. J., Hittelsman, W. N., White, R. A., andMeistrich, M. L., 1977, Relevance of density, size and DNA content of tumor cells to the lung colony assay.British Journal of Cancer,36, 659–669.
Hagmar, B., 1970, Experimental tumor metastases and blood coagulability.Acta Pathologica et Microbiologica Scandinavica, Section A, Suppl. 211.
Hagmar, B., andBoeryd, B., 1968, Effect of heparin, ε-aminocaproic acid and protamine on spontaneous metastasis formation from resectable tail-tumours.Pathologia Europea,3, 509–520.
Hagmar, B., andBoeryd, B., 1969, Distribution of intravenously induced metastases in heparin- and coumarin-treated mice.Pathologia Europea,4, 103–111.
Hagmar, B., andNorrby, K., 1973, Influence of cultivation, trypsinization and aggregation on the transplantability of melanoma B16 cells.International Journal of Cancer,11, 663–675.
Hagmar, B., Ryd, W., andErkell, L. J., 1983, Methods in metastasis research. A critical review.Invasion and Metastasis,3, 1–21.
Ohnishi, S. T., andBarr, J. K., 1978, A simplified method of quantitating protein using the biuret and phenol reagents.Analytical Biochemistry,86, 193–200.
Ryd, W., 1978, Cytological aspects on experimental tumor dissemination. Thesis, University of Göteborg.
Stackpole, C. W., Cremona, P., andKassel, R. L., 1979, Metrizamide gradient purification of mouse tumor cells.Journal of Immunological Methods,30, 231–243.
Wiepjes, E. J., andProp, F. J. A., 1970, Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse.Experimental Cell Research,61, 451–454.
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Erkell, L.J., Ryd, W. & Hagmar, B. Purity and transplantation properties of tumor cell suspensions. Clin Exp Metast 3, 1–5 (1985). https://doi.org/10.1007/BF01758949
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DOI: https://doi.org/10.1007/BF01758949