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Journal of Muscle Research & Cell Motility

, Volume 10, Issue 4, pp 326–330 | Cite as

Functional aspects of calcium transport in sarcoplasmic reticulum vesicles derived from frog skeletal muscle treated with saponin

  • G. Fanó
  • S. Belia
  • S. Fulle
  • P. Angelella
  • F. Panara
  • V. Marsili
  • R. Pascolini
Papers

Summary

To study the physiological aspects of the excitation-contraction cycle, saponin (10–100Μg ml−1) was used as a skinning agent on muscle and sarcotubular vesicles derived from fast muscles (sartorius and tibialis anterior) ofRana esculenta.

The vesicles showed similar Ca2+-ATPase activity and similar protein profiles carried out by SDS-PAGE. Calcium transport in untreated vesicles and those treated with different concentrations of saponin seemed to have the same quantitative and qualitative parameters if the saponin was used in a range between 10 and 50Μg ml−1.

Our results confirm that saponin may be considered to be a valid skinning agent for the external membranes of fast skeletal muscles.

Keywords

Calcium Skeletal Muscle ATPase Activity Sarcoplasmic Reticulum Saponin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1989

Authors and Affiliations

  • G. Fanó
    • 1
  • S. Belia
    • 1
  • S. Fulle
    • 1
  • P. Angelella
    • 1
  • F. Panara
    • 1
  • V. Marsili
    • 1
  • R. Pascolini
    • 2
  1. 1.Istituto di Biologia CellulareUniversita' degli Studi di PerugiaPerugiaItaly
  2. 2.Istituto di Anatomia ComparataUniversita' degli Studi di PerugiaPerugiaItaly

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