Summary
The glycocalyx of absorptive cells in large intestinal hyperplastic polyp was characterized histochemically at the electron microscope level by the use of the Alcian Blue pH2.5 and high iron diamine techniques with the aim of comparing their ability in preserving the fine reticular network of the structure. Both the reagents stained glycocalyx, indicating the presence of sulphated acidic glycoconjugates. However, they showed different degrees of condensation of the reactive sites. Alcian Blue preserved its filamentous appearance better.
Similar content being viewed by others
References
ARTHUR, J. F. (1968) Structure and significance of metaplastic nodules in the rectal mucosa.J. Clin. Pathol. 21, 735–43.
COSTERTON, J. W. & IRVIN, R. T. (1981) The bacterial glycocalyx in nature and disease.Amer. Rev. Microbiol. 35, 299–324.
FILIPE, M. I. (1979) Mucins in the human gastrointestinal epithelium. A review.Invest. Cell Pathol. 2, 195–216.
HASCALL, G. K. (1980) Cartilage proteoglycans: comparison of sectioned and spread whole molecules.J. Ultrastruct. Res. 70, 369–75.
JASS, J. R., FILIPE, M. I., ABBAS, S., FALCON, C. A. J., WILSON, Y. & LOVELL, D. (1984) A morphologic and histochemical study of metaplastic polyps of the colorectum.Cancer 53, 510–15.
KARNOVSKY, M. J. (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy.Proc. IIth Am. Soc. Cell Biol., New Orleans, Louisiana, Abstr. 284, p. 146.
KAYE, G. I., FENOGLIO, C. M., PASCAL, R. R. & LANE, N. (1973) Comparative electron microscopic features in normal hyperplastic and adenomatous human colonic epithelium. Variations in cellular structure relative to the process of epithelial differentiation.Gastroenterology 64, 926–45.
LUFT, J. H. (1971) Ruthenium red and violet. II. Fine structural localization in animal tissues.Anat. Rec. 171, 369–416.
MALCHIODI ALBEDI, F., CASSANO, A. M., CIARALLI, F., DONELLI, G., GIULIANI, A., MINGAZZINI, P. & MARINOZZI, V. (1988) Influence of cetylpyridinium chloride on the ultrastructural appearance of sulphated glycosaminoglycans in human colonic mucosa.Histochemistry 89, 397–401.
MALCHIODI ALBEDI, F., CASSANO, A. M., CIARALLI, F., TARUSCIO, D. & DONELLI, G. (1989) Ultrastructural identification of sulphated glycoconjugates in the Golgi apparatus in human colonic absorptive cells.Histochemistry, in press.
REALE, E., LUCIANO, L. & SPITZNAS, M. (1986) Histochemical demonstration of hyaluronic acid molecules by Alcian Blue.Histochem. J. 18, 306–16.
RINEHART, J. F. & ABUL HAJ, S. K. (1951) An improved method for histological demonstration of acid mucopolysaccharides in tissues.Arch. Pathol. 82, 189–94.
SCOTT, J. E. & ORFORD, C. R. (1981) Dermatan sulphate-rich proteoglycan associates with rat tail tendon collagen at the d band in the gap region.Biochem. J. 197, 213–16.
SCOTT, J. E., QUINTARELLI, G. & DELLOVO, M. C. (1964) The chemical and histochemical properties of Alcian Blue. I. The mechanism of Alcian Blue staining.Histochemistry 4, 73–85.
SPICER, S. S. (1965) Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–34.
SPICER, S. S., HARDIN, J. H. & SETSER, M. E. (1978) Ultrastructural visualization of sulphated complex carbohydrates in blood and epithelial cells with the high iron diamine procedure.Histochem. J. 10, 435–52.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Albedi, F.M., Ciaralli, F., Taruscio, D. et al. Ultrastructure of the absorptive cell glycocalyx in hyperplastic colonic polyps after staining with Alcian Blue and high iron diamine. Histochem J 21, 285–288 (1989). https://doi.org/10.1007/BF01757181
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01757181