Clinical & Experimental Metastasis

, Volume 9, Issue 2, pp 105–117 | Cite as

Interaction of B-cell hybridomas with fibroblast or hepatocyte monolayersin vitro and their metastatic behaviourin vivo

  • S. Verhaegen
  • H. Verschueren
  • J. Brissinck
  • D. Van Hecke
  • D. Dekegel
  • P. De Baetselier
Research Papers
  • 23 Downloads

Abstract

Using anin vitro monolayer assay (MIA) we analyzed the invasive behaviour of a panel of B-cell hybridomas prepared by the fusion of non-invasive, non-metastatic NSO plasmacytoma cells and normal murine B-cells. Interaction of these hybridomas with fibroblast-like monolayers consisted mostly of adhesion on top of the monolayers, whereas only a fraction of these cells penetrated through the monolayer. This is in sharp contrast with the highly invasive properties displayed by T-cell hybridomas. Whereas T-cell hybridomas highly infiltrated monolayers of rat hepatocyrein vitro, B-cell hybridomas neither adhered to nor infiltrated hepatocyte monolayers. We found a good correlation between the degree of adhesion of B-cell hybridomas to fibroblast-like mono-layers and their metastatic capabilities upon i.v. injection into syngeneic animals. Unlike T-cell hybridomas which formed diffuse metastasis in liver and spleen, B-cell hybridomas generated nodular metastatic lessions. When normal LPS-stimulated B-lymphocytes were tested in the fibroblast-MIA, only part of the population infiltrated the monolayers. This again contrasts with T-lymphocytes where a majority of the cells penetrated through the monolayers. These results suggest that (i) B-lymphocytes express invasive properties, albeit to a lesser extent than T-lymphocytes, (ii) non-invasive B-lymphoma cells can acquire invasiveness following cell fusion with a normal B-cell, (iii) these invasive properties contribute to the malignancy of the hybridomas when tested in recipient animals.

Keywords

Public Health Cancer Research Sharp Contrast Cell Fusion Anin 

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Copyright information

© Rapid Communications of Oxford Ltd 1991

Authors and Affiliations

  • S. Verhaegen
    • 1
  • H. Verschueren
    • 2
  • J. Brissinck
    • 1
  • D. Van Hecke
    • 1
  • D. Dekegel
    • 2
  • P. De Baetselier
    • 1
  1. 1.Dienst Algemene Biologie, Instituut voor Moleculaire BiologieVrije Universiteit BrusselSint-Genesius-RodeBelgium
  2. 2.Pasteur Instituut van BrabantBrusselsBelgium

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