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Cancer Immunology, Immunotherapy

, Volume 33, Issue 3, pp 165–170 | Cite as

Two monoclonal antibodies against small-cell lung cancer show existence of synergism in binding

  • Shin'ichi Saito
  • Tamotsu Inoue
  • Ichiro Kawase
  • Hideki Hara
  • Yoshiro Tanio
  • Isao Tachibana
  • Seiji Hayashi
  • Masatoshi Watanabe
  • Machiko Matsunashi
  • Tadashi Osaki
  • Tomiya Masuno
  • Susumu Kishimoto
Original articles

Summary

Murine IgG1 monoclonal antibodies (mAbs), ITK-2 and ITK-3, were generated against a small-cell lung cancer (SCLC) cell line. Enzyme-linked immunosorbent assay using a variety of established cell lines as substrates, immunoperoxidase staining of freshly frozen tissue sections, and fluorescence-activated cell sorter analysis of peripheral blood leukocytes showed that these mAbs recognize a part of the SCLC-associated cluster 1 antigen. In immunoprecipitation studies, both ITK-2 and ITK-3 bound to a 145-kDa glycoprotein of SCLC cell membrane extracts, as did MOC-1 and NKH-1, which both recognize the cluster 1 antigen. However, because the binding of125I-labeled ITK-2 to SCLC cells was not inhibited by MOC-1 or NKH-1, the binding site of ITK-2 on SCLC cells appeared to be different from that of either MOC-1 or NKH-1. Unexpectedly, binding of125I-labeled ITK-2 to SCLC cells increased in the presence of ITK-3. This ITK-3-induced increase in ITK-2 binding was due partly to an increase in the number of binding sites for ITK-2 on SCLC cells. Addition of ITK-3 may, therefore, improve the effectiveness of ITK-2-based tumor detection or therapy.

Key words

Monoclonal antibodies Small-cell lung cancer Synergy of mAb binding 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Shin'ichi Saito
    • 1
  • Tamotsu Inoue
    • 2
  • Ichiro Kawase
    • 1
  • Hideki Hara
    • 1
  • Yoshiro Tanio
    • 1
  • Isao Tachibana
    • 1
  • Seiji Hayashi
    • 1
  • Masatoshi Watanabe
    • 1
  • Machiko Matsunashi
    • 1
  • Tadashi Osaki
    • 1
  • Tomiya Masuno
    • 1
  • Susumu Kishimoto
    • 1
  1. 1.The Third Department of Internal MedicineOsaka University School of MedicineFukushima-ku, OsakaJapan
  2. 2.Faculty of Health and Sport ScienceOsaka UniversityToyonaka, OsakaJapan

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