Abstract
Human colonic cancer cells (HT-29, 10 7 cells/dose) were injected subcutaneously between the scapulae of 19 severe combined immunodeficient (SCID) mice. After 19 days, large tumours had developed in 18 out of the 19 animals and the mice were then killed. Metastases were detected in the lungs of 16 animals but not in other organs investigated. Surgical removal of the primary tumour in another group of five animals led to a prolonged survival and further growth of metastases in the lungs. HT-29 injection into the tail vein (n=5)resulted in colonization of the lungs. The tumours that developed in the animals were signet cell carcinomas; these forms are not seen in HT-29 cells in culture. Glycoconjugate expression of the tumours was assessed using several lectins. In many cases the results indicated a stability of lectin-binding patterns from cell culture conditions to implantation into the SCID mice. This was true for the lectin Helix pomatia agglutinin (HPA), the binding of which is associated with a high metastatic potential in some human tumours, including colon cancer. All the primary tumours and metastases were HPA positive. This xenograft tumour model seems to be a clinically relevant system for the study of glycoconjugate expression in human colon cancer cells and their metastases.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fidler IJ, 1991, Cancer metastasis.Br Med Bull,47, 157–7.
Hart IR, Goode NR and Wilson RE, 1989, Molecular aspects of the metastatic cascade.Biochim Biophys Acta,998, 65–84.
Hart IR and Saini A, 1992, Biology of tumour metastasis.Lancet,339, 1453–61.
Van Roy F and Mareel M, 1992, Tumour invasion: effects of cell adhesion and motility.Trends Cell Biol,2, 163–9.
Goldrosen MH, 1980, Murine colon adenocarcinoma.Cancer 45, 1223–8.
Bresalier RS, Raper SE, Hujanen ES and Kim YS, 1987, A new animal model for human colon cancer metastasis.Int J Cancer,39, 625–30.
Wagner HE, Toth CA, Steele GD and Thomas P, 1992, Metastatic potential of human colon cancer cell lines: relationship to cellular differentiation and carcinoembryonic antigen production.Clin Exp Metastasis,10, 25–31.
Radinsky R, 1993, Paracrine growth regulation of human colon carcinoma organ-specific metastasis.Cancer Metastasis Rev,12, 345–61.
Sekikawa K, Arends JW, Verstijnen CPHJ,et al. 1988, Influence of implantation site on growth, antigen expression and metastatic potential of human colonic cancer HT29 and 5583 xenografts in nude mice.Invasion Metastasis,8, 238–52.
Inohara H, Matsunaga T and Nomura T, 1992, Growth and metastasis of fresh human benign and malignant tumors in the head and neck regions transplanted into scid mice.Carcinogenesis,13, 845–9.
Mueller BM and Reisfeld RA, 1991, Potential of the scid mouse as a host for human tumors.Cancer Metastasis Rev,10, 193–200.
Wang M and Stearns ME, 1991, Isolation and characterization of PC-3 human prostatic tumor sublines which preferentially metastasize to select organs in S.C.I.D. mice.Differentiation,48, 115–25.
Smets LA and van Beek WB, 1984, Carbohydrates of the tumour cell surface.Biochim Biophys Acta,738, 237–49.
Nicholson GL, 1984, Cell surface molecules and tumour metastasis.Exp Cell Res,150, 3–22.
Altevogt P, Fogel M, Cheingsong-Popov R,et al. Different patterns of lectin binding and cell surface sialylation detected on related high- and low-metastatic tumour lines.Cancer Res,43, 5138–44.
Alam SM, Whitford P, Cushley W, George WD and Campbell AM, 1990, Flow cytometric analysis of cell surface carbohydrates in metastatic human breast cancer.Br J Cancer,62, 238–42.
Brooks SA and Leathem AJC, 1991, Prediction of lymph node involvement in breast cancer by detection of altered glycosylation in the primary tumour.Lancet,338, 71–4.
Fenlon S, Ellis IO, Bell J,et al. 1987, Helix pomatia and Ulex europeus lectin binding in human breast carcinoma.J Pathol,152, 169–76.
Fukutomi T, Itabashi M, Tsugane S,et al. 1989, Prognostic contribution of Helix pomatia and carcinoembryonic antigen staining using histochemical techniques in breast carcinomas.Jpn J Clin Oncol,19, 127–34.
Leathem AJ and Brooks SA, 1987, Predictive value of lectin binding on breast-cancer recurrence and survival.Lancet,i, 1054–6.
Kakeji Y, Tsujitami S, Mori M, Maehara Y and Sugimachi K, 1991,Helix pomatia binding activity is a predictor of survival time for patients with gastric carcinoma.Cancer,68, 2438–42.
Yoshida Y, Okamura T and Shirakusa T, 1993, An immunohistochemical study of Helix pomatia agglutinin binding on carcinomas of the esophagus.Surgery, Gynecol Obstet,177, 632.
Ikeda Y, Mori M, Adachi Y, Matsushima T and Sugimachi K, 1994, Prognostic value of the histochemical expression ofHelix pomatia agglutinin in advanced colorectal cancer.Dis Colon Rectum,37, 181–4.
Schumacher U, Higgs D, Loizidou M,et al. 1994,Helix pomatia agglutinin binding is a useful prognostic indicator in colorectal carcinomas.Cancer, in press.
Schumacher U, Kretzschmar H, Brooks S and Leathem A, 1992,Helix pomatia lectin binding of brain metastases originating from breast cancer.Pathol Res Practice,188, 284–6.
Schumacher U, Horny HP, Welsch U and Kaiserling E, 1989, Lectin-binding studies of a human skin mastocytoma.Histochem J,21, 44–6.
Fidler IJ, 1991, Orthotopic implantation of human colon carcinomas into nude mice provides a valuable model for the biology and therapy of metastasis.Cancer Metastasis Rev,10, 229–43.
Fu X, Besterman JM, Monosov A and Hoffman RM, 1991, Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens.Proc Nat Acad Sci USA 88, 9345–9.
Brooks S and Leathem AJ, 1989,Helix pomatia lectin binding to metastases originating from primary carcinoma of the breast.J Pathol,157, 164A.
Shiraishi T, Atsumi S and Yatani R, 1992, Comparative study of prostatic carcinoma bone metastasis among Japanese in Japan and Japanese Americans and whites in Hawai.Adv Exp Med Biol,324, 7–16.
Strous GJ and Dekker J, 1992, Mucin-type glycoproteins.Crit Rev Biochem Mol Biol,27, 57–92.
Matsumura Y and Tarin D, 1992, Significance of CD 44 gene products for cancer diagnosis and disease evaluation.Lancet,340, 1053–8.
Wilson JR and Weiser MM, 1992, Colonic cancer cell (HT29) adhesion to laminin is altered by differentiation: adhesion may involve galactosyltransferase.Exp Cell Res,201, 330–4.
Kahn HJ, Brodt P and Baumal R, 1988, Lectin binding by liver and lung metastasizing variants of the murine Lewis lung carcinoma.Am J Pathol,132, 330–4.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schumacher, U., Adam, E., Flavell, D.J. et al. Glycosylation patterns of the human colon cancer cell line HT-29 detected byHelix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in SCID mice. Clin Exp Metast 12, 398–404 (1994). https://doi.org/10.1007/BF01755883
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01755883