Clinical & Experimental Metastasis

, Volume 12, Issue 3, pp 231–237 | Cite as

A murine model for bone marrow metastasis established by an i.v. injection of C-1300 neuroblastoma in A/J mice

  • Mayumi Iwakawa
  • Koichi Ando
  • Haruo Ohkawa
  • Sachiko Koike
  • Yu-jau Chen


A reproducible tumor model for bone marrow metastasis has been developed by an injection of murine C-1300 neuroblastoma (C-1300 NB) cells into the tail vein of syngeneic A/J mice. The animals died with liver metastases at 18–21 days after an injection of 105 tumor cells and often had bone marrow metastasis in the femur. N-methylformamide (NMF), a maturational agent, was administered to inhibit liver metastases and to extend survival in mice with advancing bone metastasis. Histological examination of bone marrow metastasis, demonstrated lesions varying from a few small colonies of C-1300 NB cells either in metaphysis or diaphysis to large foci replacing normal hematopoietic bone marrow, simultaneously invading epiphysis or cortex of bone as bone metastasis. This assay demonstrated the ability to detect neuroblastoma cells in the bone marrow histologically and could determine bone marrow TD50 by extraction of bone marrow cells after treatment with various doses of drug. Fifty per cent of mice injected with cyclophosphamide (CY) developed bone marrow metastasis without liver metastasis. Treatment with tamoxifen, an anti-calmodulin drug, suppressed tumor takes in the recipient mice with tamoxifen-dose-dependent fashion. This experimental system allows for investigations into the therapeutic response and biology of neuroblastoma metastases in the bone marrow.


bone marrow metastasis bone metastasis murine neuroblastoma tumor N-methylformamide 


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Copyright information

© Rapid Communications of Oxford Ltd 1994

Authors and Affiliations

  • Mayumi Iwakawa
    • 1
    • 2
  • Koichi Ando
    • 1
  • Haruo Ohkawa
    • 2
  • Sachiko Koike
    • 1
  • Yu-jau Chen
    • 2
  1. 1.Division of Clinical ResearchNational Institute of Radiological SciencesChiba-shiJapan
  2. 2.Department of Pediatric SurgeryInstitute of Clinical Medicine, University of TsukubaIbaraki-kenJapan

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