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Detection of metastasis-associated differences for receptors of glycoconjugates (lectins) in histomorphologically unchanged xenotransplants from primary and metastatic lesions of human colon adenocarcinomas

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Abstract

Clinically applicable markers for tumor progression may be uncovered by selective analysis of biochemical parameters, supposedly participating in this complex process. Owing to the importance of specific protein-carbohydrate interactions in diverse biological processes, the pattern of the receptor part in this glycobiochemical recognition system, the sugar receptors (lectins), conceivably reflects biological properties of tumor cells in glycobiochemical terms. Therefore, we established and characterized xenografts from surgically removed specimens of a human primary colon adenocarcinoma and its metastatic lesions to liver of the same patient and of a histomorphologically similar primary colon adenocarcinoma of another patient in nude mice. Xenotransplantation and subsequent glycobiochemical analysis of material from early passages with a standardized procedure had been preferred to cell culture in monolayer on account of maintenance of a higher degree of organized histotypic assembly. Despite histomorphological similarities, the sugar receptor profile revealed significant differences in tumor-tumor and tumor-metastasis comparison, especially forα -and β-galactoside-binding proteins. Tumor-metastasis differences were substantiated by a second successfully xenotransplanted pair of specimens. Comprehensive expansion of these initial data may eventually lead to desirable functional correlations with the different biological properties of histomorphologically similar primary colon adenocarcinomas and of the metastatic phenotype and to a rational development of therapeutic modalities to restrict tumor growth and spread.

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References

  1. August, D. A., Ottow, R. T., andSugarbaker, P. H., 1984, Clinical perspectives of human colorectal cancer metastasis.Cancer and Metastasis Reviews,3, 303–325.

    PubMed  Google Scholar 

  2. Blum, H., Beier, H., andGross, H. J., 1987, Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels.Electrophoresis,8, 93–99.

    Google Scholar 

  3. Bresalier, R. S., Boland, C. R., andKim, Y. S., 1984, Characteristics of colorectal carcinoma cells with high metastatic potential.Gastroenterology,87, 115–122.

    PubMed  Google Scholar 

  4. Carding, S. R., Thorpe, S. J., Thorpe, R., andFeizi, T., 1985, Transformation and growth related changes in levels of nuclear and cytoplasmic proteins antigenically related to mammalian β-galactoside-binding lectin.Biochemical and Biophysical Research Communications,127, 680–686.

    PubMed  Google Scholar 

  5. Ciesiolka, T., andGabius, H. J., 1988, An eight-to ten-fold enhancement in sensitivity for quantitation of proteins by modified application of colloidal gold.Analytical Biochemistry,168, 280–283.

    PubMed  Google Scholar 

  6. Damjanov, I., 1987, Lectin cytochemistry and histochemistry.Laboratory Investigation,57, 5–20.

    PubMed  Google Scholar 

  7. Dennis, J. W., andLaferte, S., 1987, Tumor cell surface carbohydrate and the metastatic phenotype.Cancer and Metastasis Reviews,5, 185–204.

    PubMed  Google Scholar 

  8. Feizi, T., 1985, Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens.Nature (London),314, 53–57.

    Google Scholar 

  9. Fidler, I. J., andPoste, G., 1985, The cellular heterogeneity of malignant neoplasms: implications for adjuvant chemotherapy.Seminars in Oncology,12, 207–221.

    PubMed  Google Scholar 

  10. Fidler, I. J., andBalch, C.M., 1987, The biology of cancer metastasis and implications for therapy.Current Problems in Surgery,24, 137–209.

    Google Scholar 

  11. Gabius, H. J., 1987, Endogenous lectins in tumors and the immune system.Cancer Investigation,5, 39–46.

    PubMed  Google Scholar 

  12. Gabius, H. J., 1988, Mammalian lectins: their structure and their glycobiological and glycoclinical roles.ISI Atlas of Science: Biochemistry,1, 210–214.

    Google Scholar 

  13. Gabius, H. J., 1988, Tumorlektinologie: ein Gebiet im Schnittpunkt von Zuckerchemie, Biochemie, Zellbiologie und Onkologie.Angewandte Chemie,100, 1321–1330; Tumourlectinology: at the intersection of carbohydrate chemistry, biochemistry, cell biology and oncology.Angewandte Chemie, International Edition in English,27, 1267–1276.

    Google Scholar 

  14. Gabius, H. J., 1989, Potential participation of tumor lectins in cancer diagnosis, therapy and biology.Advances in Lectin Research, in press.

  15. Gabius, H. J., 1988, Targeting of neoglycoprotein-drug conjugates to human tumor cells via endogenous lectins.Annals of the New York Academy of Sciences,507, 337–338.

    Google Scholar 

  16. Gabius, H. J., andEngelhardt, R., 1988, Sugar receptors of different types in human metastases to lung and liver.Tumor Biology,9, 21–36.

    PubMed  Google Scholar 

  17. Gabius, H. J., andNagel, G. A. (Eds), 1988,Lectins and Glycoconjugates in Oncology (Heidelberg: Springer Verlag).

    Google Scholar 

  18. Gabius, H. J., andVehmeyer, K., 1988, Effect of microenvironmentand cell lineage type on carbohydrate-binding proteins of macrophage-like cells.Biochemistry Cell Biology,66, 1169–1176.

    Google Scholar 

  19. Gabius, H. J., Engelhardt, R., Rehm, S., andCramer, F., 1984, Biochemical characterization of endogenous carbohydrate-binding proteins from spontaneous murine rhabdomyosarcoma, mammary adenocarcinoma, and ovarian teratoma.Journal of the National Cancer Institute,73, 1349–1357.

    PubMed  Google Scholar 

  20. Gabius, H. J., Engelhardt, R., Cramer, F., Bätge, R., andNagel, G. A., 1985, Pattern of endogenous lectins in a human epithelial tumor.Cancer Research,45, 253–257.

    PubMed  Google Scholar 

  21. Gabius, H. J., Engelhardt, R., Rehm, S., Deerberg, F., andCramer, F., 1986, Differences in the pattern of endogenous lectins from spontaneous rat mammary tumors.Tumour Biology,7, 71–81.

    PubMed  Google Scholar 

  22. Gabius, H. J., Bodanowitz, S., andSchauer, A., 1988, Endogenous sugar-binding proteins in human breast tissue and benign and malignant breast lesions.Cancer,61, 1125–1131.

    PubMed  Google Scholar 

  23. Gunderson, L. L., Beart, R. W., andO'Connell, M. J., 1987, Current issues in the treatment of colorectal cancer.CRC Critical Reviews in Oncology/Hematology,6, 223–260.

    Google Scholar 

  24. Irimura, T., Ota, D. M., andCleary, K. R., 1987,Ulex europaeus agglutinin I-reactive high molecular weight glycoproteins of adenocarcinoma of distal colon and rectum and their possible relationship with metastatic potential.Cancer Research,47, 881–889.

    PubMed  Google Scholar 

  25. Kahn, H. J., andBaumal, R., 1985, Differences in lectin binding in tissue sections of human and murine malignant tumors and their metastases.American Journal of Pathology,119, 420–429.

    PubMed  Google Scholar 

  26. Kellokumpu, I. H., 1986, Differences in lectin reactivities of cellular glycoconjugates between primary human colorectal carcinomas and their metastases.Cancer Research,46, 4620–4625.

    PubMed  Google Scholar 

  27. Kim, Y. S., Yuan, M., Itzkowitz, S. H., Sum, Q., Kaizu, T., Palekar, A., Trump, B. F., andHakomori, S. I., 1986, Expression of LeY and extended LeY blood group-related antigens in human malignant, premalignant and nonmalignant colonic tissues.Cancer Research,46, 5985–5992.

    PubMed  Google Scholar 

  28. Lotan, R., Lotan, D., andRaz, A., 1985, Inhibition of tumor cell colony formation in culture by a monoclonal antibody to endogenous lectins.Cancer Research,45, 4349–4353.

    PubMed  Google Scholar 

  29. Lotan, R., andRaz, A., 1988, Endogenous lectins as mediators of tumor cell adhesion.Journal of Cellular Biochemistry,37, 107–117.

    PubMed  Google Scholar 

  30. Moutsatsos, I. K., Wade, M., Schindler, M., andWang, J. L., 1987, Endogenous lectins from cultured cells: nuclear localization of carbohydrate-binding protein 35 in proliferating 3T3 fibroblasts.Proceedings of the National Academy of Sciences of the USA,84, 6452–6456.

    PubMed  Google Scholar 

  31. Nicolson, G. L., 1982, Cancer metastasis: organ colonization and the cell surface properties of malignant cells.Biochimica et Biophysica Acta,695, 113–176.

    PubMed  Google Scholar 

  32. Nicolson, G. L., 1984, Cell surface molecules and tumor cell metastasis.Experimental Cell Research,150, 3–22.

    PubMed  Google Scholar 

  33. Ochs, D., 1983, Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels.Analytical Biochemistry,135, 470–474.

    PubMed  Google Scholar 

  34. Osborn, M., andWeber, K., 1982, Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: tubulin-containing structures.Methods in Cell Biology,24, 97–132.

    PubMed  Google Scholar 

  35. Schirrmacher, V., Altevogt, P., Fogel, M., Dennis, J., Waller, C. A., Barz, D., Schwartz, R., Cheingsong-Popov, R., Springer, G., Robinson, P. J., Nebe, T., Brossmer, W., Vlodavsky, I., Paweletz, N., Zimmermann, H. P., andUhlenbruck, G., 1982, Importance of cell surface carbohydrates in cancer cell adhesion, invasion and metastasis.Invasion and Metastasis,2, 313–360.

    Google Scholar 

  36. Schirrmacher, V., 1985, Cancer metastasis: experimental approaches, theoretical concepts, and impact for treatment strategies.Advances in Cancer Research,43, 1–73.

    PubMed  Google Scholar 

  37. Schoentag, R., Primus, F. J., andKuhns, W., 1987, ABH and Lewis blood group expression in colorectal carcinoma.Cancer Research,47, 1695–1700.

    PubMed  Google Scholar 

  38. Seve, A. P., Hubert, J., Bouvier, D., Bourgeois, C., Midoux, P., Roche, A. C., andMonsigny, M., 1986, Analysis of suger-binding sites in mammalian cell nuclei by quantitative flow microfluorometry.Proceedings of the National Academy of Sciences of the USA,85, 5997–6001.

    Google Scholar 

  39. Spremulli, E. N., andDexter, D. L., 1983, Human tumor cell heterogeneity and metastases.Journal of Clinical Oncology,1, 496–509.

    PubMed  Google Scholar 

  40. Tarin, D., 1985, Clinical and experimental studies on the biology of metastasis.Biochimica et Biophysica Acta,780, 227–235.

    PubMed  Google Scholar 

  41. Vehmeyer, K., Hajto, T., andGabius, H. J., 1988, Immunomodulation by endogenous β-galactoside-specific lectins. In:Lectins and Glycoconjugates in Oncology, edited by H. J. Gabius and G. A. Nagel (Heidelberg: Springer), pp. 207–211.

    Google Scholar 

  42. Warren, L., Fuhrer, J. P., andTusgynski, G. P., 1978, Glycopeptide changes and malignant transformation: a possible role for carbohydrates in malignant behavior.Biochimica et Biophysica Acta,516, 97–127.

    PubMed  Google Scholar 

  43. Weiss, L., 1985,Principles of Metastasis (Orlando: Academic Press).

    Google Scholar 

  44. Yamamura, M., Hamada, Y., Kogata, M., Kiba, M., Uetsuji, S., Yamamoto, M., andAdachi, M., 1985, Postoperative immunization with peanut agglutinin-binding glycoprotein from Lewis lung carcinoma in mice.Journal of Surgical Research,38, 39–44.

    PubMed  Google Scholar 

  45. Yogeeswaran, G., 1983, Cell surface glycolipids and glycoproteins in malignant transformation.Advances in Cancer Research,38, 289–350.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Abteilung Chemie, Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Strasse 3, D-3400, Göttingen

    Hans -Joachim Gabius & Thomas Ciesiolka

  2. Zentrum Pathologie der Universität, Robert-Koch-Strasse 40, D-3400, Göttingen

    Ekkehard Kunze

  3. Abteilung Hämatologie-Onkologie, Medizinische Universitätsklinik, Robert-Koch-Strasse 40, D-3400, Göttingen, Federal Republic of Germany

    Katalin Vehmeyer

Authors
  1. Hans -Joachim Gabius
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  2. Thomas Ciesiolka
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  3. Ekkehard Kunze
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  4. Katalin Vehmeyer
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Dedicated to Prof. Dr G. Uhlenbruck on the occasion of his 60th birthday.

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Gabius, H.J., Ciesiolka, T., Kunze, E. et al. Detection of metastasis-associated differences for receptors of glycoconjugates (lectins) in histomorphologically unchanged xenotransplants from primary and metastatic lesions of human colon adenocarcinomas. Clin Exp Metast 7, 571–584 (1989). https://doi.org/10.1007/BF01753816

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  • DOI: https://doi.org/10.1007/BF01753816

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