Clinical & Experimental Metastasis

, Volume 7, Issue 3, pp 353–359 | Cite as

Effects of laminin on the attachment of glioma cells to type IV collagen

  • Gillian Hunt
  • G. V. Sherbet


The interaction of tumour cells with basement membrane components is thought to be important in influencing their invasive and metastatic properties. This paper describes the effect of laminin on the attachment of radiolabelled glioma and B16 murine melanoma cells to tissue culture plastic and type IV collagen. With the exception of the non-metastatic B16 F1 variant, laminin (and fibronectin) stimulated cell attachment to tissue culture plastic. Although laminin stimulated the attachment of the B16 BL6 metastatic variant to type IV collagen, it consistently inhibited the attachment of the glioma cells under the same conditions. Laminin appeared to exert its effect by adsorption to the collagen and was not cytotoxic to the glioma cells. In contrast, fibronectin had very little effect on cell attachment to type IV collagen. One of the most unusual features of glioma is the rarity of metastasis to extraneural sites. However, the effect of laminin observed here may not be the only factor involved in the metastatic inefficiency of this tumour type.


Melanoma Melanoma Cell Glioma Cell Laminin Cell Attachment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Taylor & Francis Ltd. 1989

Authors and Affiliations

  • Gillian Hunt
    • 1
  • G. V. Sherbet
    • 1
  1. 1.Cancer Research Unit, The Medical SchoolUniversity of Newcastle upon TyneNewcastle upon TyneUK

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