Abstract
The interaction of tumour cells with basement membrane components is thought to be important in influencing their invasive and metastatic properties. This paper describes the effect of laminin on the attachment of radiolabelled glioma and B16 murine melanoma cells to tissue culture plastic and type IV collagen. With the exception of the non-metastatic B16 F1 variant, laminin (and fibronectin) stimulated cell attachment to tissue culture plastic. Although laminin stimulated the attachment of the B16 BL6 metastatic variant to type IV collagen, it consistently inhibited the attachment of the glioma cells under the same conditions. Laminin appeared to exert its effect by adsorption to the collagen and was not cytotoxic to the glioma cells. In contrast, fibronectin had very little effect on cell attachment to type IV collagen. One of the most unusual features of glioma is the rarity of metastasis to extraneural sites. However, the effect of laminin observed here may not be the only factor involved in the metastatic inefficiency of this tumour type.
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References
Brown, K. W., andParkinson, E. K., 1985, Alteration of the extracellular matrix of cultured human keratinocytes by transformation and during differentiation.International Journal of Cancer,35, 799–807.
Fligiel, S. E. G., Laybourn, K. A., Peters, B. P., Ruddon, R. W., Hiserodt, J. C., andVarani, J., 1986, Laminin production by murine melanoma cells: possible involvement in cell motility.Clinical and Experimental Metastasis,4, 259–272.
Giavazzi, R., Liotta, L., andHart, I., 1982, Laminin inhibits the adhesion of a murine tumour of macrophage origin.Experimental Cell Research,140, 315–322.
Hirano, A., Ghatak, N. R., Becker, N. H., andZimmerman, H. M., 1974, A comparison of the fine structure of small blood vessels in intracranial and retroperitoneal malignant lymphomas.Acta Neuropathology,27, 93–104.
Klebe, R. J., 1974, Isolation of a collagen-dependent cell attachment factor.Nature (London),250, 248–251.
Liotta, L. A., 1986, Tumour invasion and metastasis-role of the basement membrane.Cancer Research,46, 1–7.
Mandybur, T. I., Liwnicz, B. H., andWechsler, W., 1984, Disseminated (metastatic) tumours in nude mice produced by intravenous injection of cells of human and non-human neurogenic tumour lines. ActaNeuropathology,63, 203–209.
McKeever, P. E., Fligiel, S. E. G., Varani, J., Hudson, J. L., Smith, D., Castle, R. L., andMcCoy, J. P., 1986, Products of cells cultured from gliomas. IV. Extracellular matrix proteins of gliomas.International Journal of Cancer,37, 867–874.
Pasquier, B., Pasquier, D., Golet, A. N., Panh, M. H., andCoudere, P., 1980, Extraneural metastases of astrocytomas and glioblastomas.Cancer,45, 112–125.
Rao, C. N., Margulies, I. M. K., andLiotta, L. A., 1984, Molecular regions mediating binding of type IV collagen to laminin.Federation Proceedings,43, 992 (Abstract).
Sherbet, G. V., 1987,The Metastatic Spread of Cancer: Reflections on its Mechanisms Based on the Malignant Glioma Model (London: Macmillan), p. 80.
Terranova, V. P., Liotta, L. A., Russo, R. G., andMartin, G. R., 1982, Role of laminin in the attachment and metastasis of murine tumour cells.Cancer Research,42, 2265–2269.
Timpl, R., Engel, J., andMartin, G. R., 1983, Laminin—a multifunctional protein of basement membranes.Trends in Biochemical Sciences,8, 207–209.
Varani, J., Carey, T. E., Fligiel, S. E. G., McKeever, P. E., andDixit, V., 1987, Tumour type-specific differences in cell-substrate adhesion among human tumour cell lines.International Journal of Cancer,39, 397–403.
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Hunt, G., Sherbet, G.V. Effects of laminin on the attachment of glioma cells to type IV collagen. Clin Exp Metast 7, 353–359 (1989). https://doi.org/10.1007/BF01753686
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DOI: https://doi.org/10.1007/BF01753686