Clinical & Experimental Metastasis

, Volume 6, Issue 4, pp 333–346 | Cite as

Metastatic instability of murine tumor metastases: Dependence on tumor type

  • John P. Volpe
  • Luka Milas
Article

Abstract

Fifteen metastatic lines derivedin vivo from three syngeneic murine tumors, the sarcomas SA-NH and SA-4020 and the hepatic carcinoma HCA-I, were assessed for their stability of metastasis formation upon isotransplantation for several successive generations in syngeneic animals. Change in the metastatic phenotype was actively encouraged by a new procedure, the artificial selection for increased or decreased metastasis formation. Metastatic instability was dependent on tumor type, with five of six lines of tumor SA-NH, one of four lines of tumor SA-4020, and possibly one of five lines of HCA-I changing in lung metastasis formation. The instability of lung metastasis formation was also assessed by analyzing changes in the variance of the lines. Concomitant with a change in metastatic potential for lung metastasis, we observed a similar change for abdominal lymph node metastasis. We also report the selection of a less metastatic line. The variance of lung metastasis increased significantly only in the SA-NH lines. The instability of metastasis formation was attributed to genetic instability of metastatic cell lines.

Keywords

Lymph Node Metastasis Sarcoma Tumor Type Lung Metastasis Genetic Instability 

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • John P. Volpe
    • 1
  • Luka Milas
    • 1
  1. 1.Department of Experimental RadiotherapyThe University of Texas M. D. Anderson Hospital and Tumor Institute at HoustonHoustonUSA

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