Clinical & Experimental Metastasis

, Volume 6, Issue 4, pp 333–346 | Cite as

Metastatic instability of murine tumor metastases: Dependence on tumor type

  • John P. Volpe
  • Luka Milas


Fifteen metastatic lines derivedin vivo from three syngeneic murine tumors, the sarcomas SA-NH and SA-4020 and the hepatic carcinoma HCA-I, were assessed for their stability of metastasis formation upon isotransplantation for several successive generations in syngeneic animals. Change in the metastatic phenotype was actively encouraged by a new procedure, the artificial selection for increased or decreased metastasis formation. Metastatic instability was dependent on tumor type, with five of six lines of tumor SA-NH, one of four lines of tumor SA-4020, and possibly one of five lines of HCA-I changing in lung metastasis formation. The instability of lung metastasis formation was also assessed by analyzing changes in the variance of the lines. Concomitant with a change in metastatic potential for lung metastasis, we observed a similar change for abdominal lymph node metastasis. We also report the selection of a less metastatic line. The variance of lung metastasis increased significantly only in the SA-NH lines. The instability of metastasis formation was attributed to genetic instability of metastatic cell lines.


Lymph Node Metastasis Sarcoma Tumor Type Lung Metastasis Genetic Instability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • John P. Volpe
    • 1
  • Luka Milas
    • 1
  1. 1.Department of Experimental RadiotherapyThe University of Texas M. D. Anderson Hospital and Tumor Institute at HoustonHoustonUSA

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