Summary
We have determined the nucleotide sequences of 5 S ribosomal RNAs from two marineVibrio species,V. fluvialis andV. parahaemolyticus. Comparisons among these and the 5S rRNA sequence fromV. cholerae indicate homologies in excess of 92%. Comparisons are also made with the 5S rRNA sequences from two other members of the family Vibrionaceae.
Résumé
Nous avons déterminé les séquences nucléotidiques de l'ARN ribosomique 5S chez deux espèces deVibrio, V. fluvialis etV. parahaemolyticus. La comparaison de ces deux séquences entre elles et avec celle deV. cholerae montre une homologie supérieure à 92%. Il a également été procédé à une comparaison avec les séquences du r-ARN de deux autres membres de la famille de Vibrionaceae.
Resumen
Se han determinado las secuencias de nucleótidos correspondientes a 5S-rRNA ribosómico de dos especies deVibrio marinas:V. fluvialis yV. parahaemolyticus. Comparaciones entre estas y la correspondiente secuencia 5S-rRNA deV. cholerae indican una homología superior al 92%. También se han comparado las secuencias 5S-rRNA de dos otros miembros de la familia Vibrionáceas.
References
D'Alessio, J. M. (1982) RNA Sequencing. In:Gel Electrophoresis of Nucleic Acids (Ed. by D. Rickwood & B. D. Hames) IRL Press, London.
Donis-Keller, H., Maxam, A. M. & Gilbert, W. (1977) Mapping adenines, guanines and pyrimidines in RNA.Nucleic Acids Research,4, 2527–2538.
Hansen, J. N. (1981) Use of solubilizable acrylamide disulfide gels for isolation of DNA fragments suitable for sequence analysis.Analytical Biochemistry,116, 146–151.
Kirby, K. S. (1956) A new method for the isolation of ribonucleic acids from mammalian tissues.Biochemistry Journal,64, 405–408.
Luehrsen, K. & Fox, G. E. (1981) The nucleotide sequence ofBeneckea harveyi 5SrRNA.Journal of Molecular Evolution,17, 52–55.
MacDonell, M. T. & Colwell, R. R. (1984) Identical 5S rRNA nucleotide sequences ofV. cholerae strains representing temporal, geographical and ecological diversity.Applied and Environmental Microbiology,48, 119–121.
Randerath, K. & Randerath, E. (1967) Thin-layer separation methods for nucleic acid derivatives.Methods in Enzymology,12A, 323–347.
Woese, C. R., Pribula, C. D., Fox, G. E. & Zablen, L. B. (1975) Nucleotide sequence of the ribosomal RNA from a Photobacterium.Journal of Molecular Evolution,5, 35–46.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MacDonell, M.T., Colwell, R.R. Nucleotide sequences of 5S rRNA from twoVibrio spp., V.fluvialis andV. parahaemolyticus . World J Microbiol Biotechnol 1, 97–102 (1985). https://doi.org/10.1007/BF01748158
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01748158