Skip to main content
Log in

Extracellular enzymes of some black aspergilli in Singapore

Enzymes exo-cellulaires de certains aspergilli noirs isolés à Singapour

Enzimas extracelulares de algunos aspergillus negros de Singapur

  • Research Papers
  • Published:
MIRCEN journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

Ten strains ofAspergillus niger and one strain ofA. awamori showed proteolytic activity and amylolytic and cellulolytic activities were observed in all the strains except inA. niger isolated from rice grains.Aspergillus awamori was shown to be the best producer of amylolytic enzymes but poorest for cellulases. All the strains were shown to be good producers of proteolytic enzymes while the strain from rubber wood seemed to be the best cellulase-producing strain. Both single conidium and hyphal tip inoculation techniques gave similar results. Differences in the levels of extracellular enzymes produced by similar strains were also observed using the two different techniques.

Résumé

Dix souches d'Aspergillusniger et une d'A.awamori ont été étudiées. Toutes ces souches ont une activité protéolytique et toutes, sauf une souche d'A.niger isolée à partir de grains de riz, ont des activités amylolytiques et cellulolytiques. Il a été constaté qu'Aawamori est le meilleur producteur d'enzymes amylolytiques, mais le moins bon producteur de cellulases. Toutes les sourches sont bonnes productrices d'enzymes protéolytiques, et celle provenant d'un arbre à caoutchouc est la meilleure productrice de cellulase. Les techniques d'inoculation avec une conidie unique ou avec une extrémité d'hyphe donnent des résultats similaires. Les différences de niveau des enzymes exo-cellulaires produits par des souches similaires ont également été observées avec ces deux techniques différentes.

Resumen

Se han aislado diez cepas deAspergillus niger y una deA. awamori que presentan buena actividad proteolítica. Todas las cepas, excepto una deA. niger, aislado de granos de arroz, mostraron, además actividades amilolíticas y celulolíticas. Los mejores productores de enzimas celulolíticas y amilolíticos parecen ser, respectivamente, la cepa deA. niger aislada de madera de caucho yA. awamori. Aspergillus awamori es, sin embargo, el peor productor de celulasas. Se han usado dos técnicas de inoculación, a partir de una solo conidio o bien a partir de un trozo de hifa, obteniéndose resultados comparables. Utilizando estas mismas técnicas se han observado variaciones en las cantidades de enzimas excretados por cepas semejantes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barbesgaard, P. (1977) Industrial enzymes produced by members of the genusAspergillus. In:Genetics and Physiology of Aspergillus (ed. by Smith R. E. & Pateman J. A.), PP. 391–404. Academic Press, London.

    Google Scholar 

  • Charney, W. (1984) Microbial transformation.7th International Biotechnology Symposium, pp. 33–35. New Delhi, India.

  • Cohen, B. L. (1977) The proteases of Aspergilli. In:Genetics and Physiology of Aspergillus, (ed. by Smith R. E. & Pateman J. A.), pp. 281–292. Academic Press, London.

    Google Scholar 

  • Eswaran, S. (1978)Studies on the growth of A. nigerstrains on tapioca starch. PhD dissertation. Department of Botany, University of Singapore.

  • Feniksova, R. V. & Shilova, A. A. (1960) Saccharification of starch with mold enzymes. USSR Patent No. 127, 632. April 12.

  • Hankin, L. & Anagnostakis, S. L. (1975) The use of solid media for detection of enzyme production by fungi.Mycologia,67, 597–607.

    Google Scholar 

  • Kurtzman, C. P. (1983) Fungi — sources of food, fuel, and biochemicals.Mycologia,75, 374–382.

    Google Scholar 

  • Le Mense, E. H., Corman, J., van Lanen, J. M., & Langlykke, A. F. (1947) Production of mould amylases in submerged culture.Journal of Bacteriology,54, 149–159.

    Google Scholar 

  • Lowe, D. A. & Elander, R. P. (1983) Contribution of mycology to the antibiotic industry.Mycologia,75, 361–373.

    Google Scholar 

  • Reese, E. T. & Downing, M. H. (1951) Activity of the aspergilli on cellulose, cellulose derivatives and wool.Mycologia,43, 16–28.

    Google Scholar 

  • Ryu, D. D. Y. & Mandels, M. (1980) Cellulases: biosynthesis and applications.Enzyme Microbial Technology,2, 91–102.

    Google Scholar 

  • Saddler, J. N., Hogan, C., Chan, M. K.-H. & Louis-Seize, G. (1982) Ethanol fermentation of enzymatically hydrolysed pretreated wood fractions usingTrichoderma cellulases,Zymomonas mobilis andSaccharomyces cerevisiae.Canadian Journal of Microbiology,28, 1311–1319.

    Google Scholar 

  • Smith, R. E. (1977) Rapid tube test for detecting fungal cellulase production.Applied and Environmental Microbiology,33, 980–981.

    Google Scholar 

  • Taylor, M: J. & Richards, T. (1979) Applications of microbial enzymes in food systems and in biotechnology. In:Advances in Applied Microbiology (ed. by Perlman D.) vol. 25, pp. 7–35. Academic Press, New York.

    Google Scholar 

  • Thomas, A. (1977) The genusAspergillus and biodeterioration. In:Genetics and Physiology of Aspergillus, (ed. by Smith R. E. & Pateman J. A.) pp. 453–479. Academic Press, London.

    Google Scholar 

  • White, L. W., Siu, R. G.H. & Reese, E. T. (1948) The black Aspergilli in relation to cellulosic substrate.Bulletin of the Torrey Botanical Club,75, 604–632.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lim, G., Khew, E. & Yeoh, H.H. Extracellular enzymes of some black aspergilli in Singapore. World J Microbiol Biotechnol 1, 55–61 (1985). https://doi.org/10.1007/BF01748154

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01748154

Keywords

Navigation