Klinische Wochenschrift

, Volume 44, Issue 23, pp 1366–1371 | Cite as

Cytologische und cytogenetische Untersuchungen an homologen und heterologen Lymphocyten-Mischkulturen

  • R. Fischer
  • A. Gropp
  • H. Geiger
Originalien

Zusammenfassung

In Mischkulturen von menschlichen Blutlymphocyten verschiedener Spender treten auch in Abwesenheit von Phytohämagglutinin oder von spezifischen Antigenen aktivierte, blastenartige und proliferierende Zellen auf. In Erweiterung bisher vorliegender Befunde wurden Untersuchungen über die gegenseitige Lymphocytenstimulation bei der Kultivierung von Blutzellen anisogenetischer menschlicher Spender und von Milzlymphocyten der Maus und des Meerschweinchens durchgeführt. Die Ergebnisse zeigen, daß die reziproke Lymphocytenaktivierung nicht artbegrenzt ist, sondern auch in Mischkulturen der verschiedenen intra- und interspezifischen Versuchsgruppierungen vorhanden ist. Die in den homologen und heterologen Lymphocyten-Mischkulturen in wechselnder Zahl auftretenden blastenartig transformierten und proliferierenden Zellen zeigen in ihren cytologischfärberischen Eigenschaften eine weitgehende Übereinstimmung mit den unter Phytohämagglutininbzw. spezifischer Antigen-Stimulation nachweisbaren aktivierten Lymphocyten. Cytogenetische Untersuchungsverfahren erlauben mit Hilfe der „Chromosomen-Marker-Technik“ anhand einfacher chromosomaler Erkennungsmerkmale eine Analyse der jeweiligen Herkunft der blastenartigen Zellen und liefern den Beleg dafür, daß die Lymphocyten der Spender-Partner sich gegenseitig zu stimulieren vermögen.

Summary

Blastoid transformation and proliferation of lymphocytes can be observed in mixed cultures of human white blood cells of different donors. This transformation occurs in the absence of phytohaemagglutinin or specific antigens. Observations on reciprocal activation of anisogeneic human lymphocytes of peripheral blood are supplemented by results on mixed cultures of spleen lymphocytes from the mouse and guinea-pig. It should be stressed that reciprocal lymphocytic activation is not limited to a species, but occurs in intra- and interspecific combinations from different donors. This effect can be observed in animals which show no or only a conditioned phytohaemagglutinin-response of lymphocytes. The blastoid cells occuring in homologous and heterologous mixed lymphocyte cultures display the same cytologic characteristics as the human blastoid cells induced by phytohaemagglutinin or antigenic stimulation. Cytogenetic methods provide means of identification of the individual origin of blastoid cells by using simple cytogenetic (chromosomal) markers in the different sexes or species of the donors. Thus it can be stated that mixed lymphocytic reaction leads to a mutual or two-way activation of the lymphocytes of both donors. The mixed lymphocytic reaction seems to represent a immunocytological reaction based on a membrane-bound antigen-antibody interaction of competent lymphocytes.

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Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • R. Fischer
    • 1
  • A. Gropp
    • 1
  • H. Geiger
    • 1
  1. 1.Pathologisches Institut der Universität BonnGermany

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