Summary
A polyclonal antiserum to protein kinase C has been used to study the distribution of the enzyme antigenic sites in rat retina. The results indicate that the kinase is concentrated in photoreceptor outer segments as well as in the outer and inner plexiform layers. In identified components of retinal neuronal circuits, the kinase immunoreactivity is present in photoreceptor presynaptic terminals, in bipolar cell dendrites and axons, and probably in bipolar cell presynaptic terminals impinging on retinal ganglion cell dendrites. Thus, protein kinase C is positioned to play a role in specialized compartments of photoreceptor membrane and at both pre- and postsynaptic levels in the function of retinal neuronal circuits. Label in the nucleus is observed in retinal ganglion cells, but not bipolar or horizontal cells and probably not in amacrine cells. A role for protein kinase C in neuronal function at the level of the cell nucleus is therefore not likely to be universal, but to be determined by the particular properties of individual neuronal types.
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BARABAN, J. M., SNYDER, S. H. & ALGER, B. E. (1985) Protein kinase C regulates ionic conductance in hippocampal pyramidal neurons: electrophysiological effects of phorbol esters.Proc. Natl. Acad. Sci. USA 82, 2538–42.
DECAMILLI, P., CAMERON, R. & GREENGARD, P. (1983) Synapsin I (protein I), a nerve terminal specific phosphoprotein. 1. Its general distribution in synapses of the central and peripheral nervous system demonstrated by immunofluorescence in frozen and plastic sections.J. Cell Biol. 96, 1337–54.
DEREIMER, S. A., STRONG, J. A., ALPERT, K. A., GREENGARD, P. A. & KACMARCK, L. K. (1985) Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C.Nature 313, 313–16.
DIVIRGILIO, F., POZZAN, T., WOLLHEIM, C. B., VINCENTI, L. M. & MELDOLESI, J. (1986) Tumor promoter phorbol myristate acetate inhibits calcium influx through voltage-gated calcium channels in two secretoty cell lines, PC12 and RINm5F.J. Biol. Chem. 261, 32–5.
FISHER, D., AUERBACH, S. & FARLEY, J. (1985) Protein kinase C reduces potassium currents and enhances calcium currents in Hermissenda type B cells.Soc. Neurosci. Abstr. 11, 788a.
GIRARD, P. R., MAZZEI, G. J., WOOD, J. G. & KUO, J. F. (1985) Polyclonal antibodies to phospholipid/calcium-dependent protein kinase and immunocytochemical localization of the enzyme in rat brain.Proc. Natl. Acad. Sci. USA 82, 3030–4.
GIRARD, P. R., MAZZEI, G. J. & KUO, J. F. (1986) Immunological quantitation of phospholipid/calcium dependent protein kinase and its fragments. Tissue levels, subcellular distribution, and ontogenetic changes in brain and heart.J. Biol. Chem. 261, 370–5.
INOUE, M. & ISAYAMA, Y. (1984) Calcium ion and phospholipid-dependent protein kinase in rod outer segment.Jpn. J. Ophthalmol. 28, 47–56.
KAPOOR, C. L. & CHADER, G. J. (1984) Endogenous phosphorylation of retinal photoreceptor outer segment proteins by calcium phospholipid-dependent protein kinase.Biochem. Biophys. Res. Commun. 122, 1397–403.
KELLEHER, D. J. & JOHNSON, G. L. (1985) Purification of protein kinase C from bovine rod outer segments.J. Cyclic Nucleotide Protein Phosphorylation Res. 10, 579–91.
KELLEHER, D. J. & JOHNSON, G. L. (1986) Phosphorylation of rhodopsin by protein kinase Cin vitro.J. Biol Chem. 261, 4749–57.
LAEMMLI, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227, 680.
NISHIZUKA, Y. (1984) Turnover of inositol phospholipids and signal transduction.Science 225, 1365–70.
OHKUBO, S., YAMADA, E., ENDO, T., ITOH, H. & HIDAKA, H. (1984) Vitamin A acid-induced activation of calciumactivated, phospholipid-dependent protein kinase from rabbit retina.Biochem. Biophys. Res. Commun. 118, 460–6.
OLMSTED, J. B. (1981) Affinity purification of antibodies from diazotized paper blots of heterogenous protein samples.J. Biol. Chem. 256, 11955–7.
RANE, S. G. & DUNLAP, K. (1986) Kinase C activator 1,2-oleoylacetylglycerol attenuates voltage-dependent calcium current in sensory neurons.Proc. Natl. Acad. Sci. USA 83, 184–8.
TOWBIN, H., STAHELIN, T. & GORDON, J. (1979) Electrophoretical transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications.Proc. Natl. Acad. Sci. USA 76, 4350.
WOOD, J. G., MCLAUGHLIN, B. J. & VAUGHN, J. (1976) Immunocytochemical localization of GAD in electron microscopic preparations of rodent CNS. InGABA in Nervous System Function, (edited by ROBERTS, E., CHASE, T. & TOWER, D.) pp. 133–49. New York: Raven Press.
WOOD, J. G., WALLACE, R. W., WHITAKER, J. N. & CHEUNG, W. Y. (1980) Immunocytochemical localization of calmodulin and a heat labile calmodulin binding protein (Cam-BP80) in rodent brain.J. Cell Biol. 84, 66–76.
WOOD, J. G., GIRARD, P. R., MAZZEI, G. J. & KUO, J. F. (1986) Immunocytochemical localization of protein kinase C in identified neuronal compartments of rat brain.J. Neurosci. 6, 2571–7.
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Wood, J.G., Hart, C.E., Mazzei, G.J. et al. Distribution of protein kinase C immunoreactivity in rat retina. Histochem J 20, 63–68 (1988). https://doi.org/10.1007/BF01746605
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DOI: https://doi.org/10.1007/BF01746605