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Quantitative Immunglobulin-Bestimmungen im Serum bei entzündlichen Leberkrankheiten

I. Normalwerte und Untersuchungen im Verlaufe der akuten Hepatitis

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Zusammenfassung

Mit der einfachen radialen Immundiffusionsmethode wurden die Serumkonzentrationen von IgG, IgA und IgM bei 100 bzw. 150 Normalpersonen bestimmt. Bei 20 Personen mit akuter Hepatitis fanden wir in der ersten Woche nach Beginn des Ikterus eine sehr starke IgM-Erhöhung bei nur mäßig vermehrtem IgG und kaum vermehrtem IgA. Innerhalb des Beobachtungszeitraumes von sechs Wochen fiel die IgM-Konzentration wieder bis an die obere Normgrenze ab, während bei zwei Patientinnen mit chronisch persistierender Hepatitis ein über viele Monate isoliert erhöhtes IgM das Fortbestehen der Hepatitis anzeigte. Der Anstieg der IgG-Konzentration bei der akuten Hepatitis war dagegen nur mäßig ausgeprägt. Die Bedeutung der durch isolierte Messung einzelner Ig-Klassen erzielten Befunde liegt in der Differenzierungsmöglichkeit zwischen den ätiologisch unterschiedlichen Gruppen entzündlicher Leberkrankheiten.

Summary

Using a radial immunodiffusion method the serum concentrations of IgG, IgA, and IgM have been determined in 100/150 normal probands. In 20 patients with acute viral hepatitis, markedly elevated IgM levels were observed during the first few weeks after the beginning of jaundice, whereas IgG and IgA were only slightly increased. In these patients the IgM levels returned to normal within six weeks. Two patients with chronic persistent hepatitis showed definitely elevated IgM serum values during observation periods of many months. — Quantitative determination of several immunoglobulins can be a helpful method for the differentiation of different types of inflammatory liver disorders.

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Literatur

  1. Altemeier, W. A., J. B. Robbins, andR. T. Smith: Quantitative studies of the immunoglobulin sequence in the response of the rabbit to a somatic antigen. J. exp. Med.124, 443 (1966).

    Google Scholar 

  2. Armstrong, A. S., andC. P. Sword: Antibody responses in experimental infections with listeria monocytogenes. J. Immunol.98, 510 (1967).

    Google Scholar 

  3. Bachmann, R., andC.-B. Laurell: Studies on the serum γ1A-globulin level. I. Healthy subjects and hypergammaglobulinemic patients. Scand. J. clin. Lab. Invest.17, 39 (1965).

    Google Scholar 

  4. Bargob, I., H. Cleve u.F. Hartmann: Immunelektrophoretische Serumunterschungen bei Lebererkrankungen. Dtsch. Arch. klin. Med.204, 708 (1958).

    Google Scholar 

  5. Beier, W., u.E. Dörner: Die Physik und ihre Anwendung in Medizin und Biologie. Leipzig: VEB Georg Thieme 1960.

    Google Scholar 

  6. Claman, H. N., andD. Merril: Hypergammaglobulinemia — the role of the immunoglobulins (γG, γA, γM). J. Allergy36, 463 (1965).

    Google Scholar 

  7. Collins-Williams, C., S. J. Tkachyk, B. Toft, andM. Moscarello: Quantitative immunoglobulin levels (IgG, IgA and IgM) in children. Int. Arch. Allergy31, 94 (1967).

    Google Scholar 

  8. Cwynarski, M. T.: Serum immunoglobulin levels in monoclonal Protein diseases. Vortrag vor dem 15. Annual Colloquium of protides of the Biological Fluids, Brügge 1967.

  9. Elisberg, E. L., Wm. H. Wood jr., andJ. A. Bellanti: Serologic properties in human murine typhus. Fed. Proc.26, 420 (1967).

    Google Scholar 

  10. Fahey, J. L., andE. M. McKelvey: Quantitative determination of serum immunoglobulins in antibodyagar plates. J. Immunol.94, 84 (1965).

    Google Scholar 

  11. Feissli, S., G. Forster, G. Laudahn, E. Schmidt u.F. W. Schmidt: Normalwerte und Alterung von Hauptketten-Enzymen im Serum. Klin. Wschr.44, 390 (1966).

    Google Scholar 

  12. Finkelstein, M. S., andJ. W. Uhr: Antibody formation: V. The avidity of γM and γσ guinea pig antibodies to bacteriophage. ΦX 174. J. Immunol.97, 565 (1966).

    Google Scholar 

  13. Fitch, F. W., andR. W. Wissler: The histology of antibody production. In:M. Samter andH. L. Alexander, Immunological diseases. Boston: Little, Brown & Co. 1965.

    Google Scholar 

  14. Gleichmann, E., u.H. Deicher: Differenzierung der Hyper-γ-Globulinämie bei entzündlichen Lebererkrankungen. Klin. Wschr.45, 684 (1967).

    Google Scholar 

  15. Gleichmann, E., u.H. Deicher: Quantitative Immunglobulinbestimmungen im Serum bei entzündlichen Leberkrankheiten. II. Chronisch entzündliche Lebererkrankungen. Klin. Wschr. (im Druck).

  16. Hartmann, L., P. Burtin, P. Grabar etR. Fauvert: L'analyse immuno-électrophorétique des sérums de cirrhoses. C. R. Acad. Sci. (Paris)243, 1937 (1956).

    Google Scholar 

  17. Heimburger, N., K. Heide, H. Haupt u.H. E. Schultze: Bausteinanalysen von Humanserumproteinen. Clin. chim. Acta10, 293 (1964).

    Google Scholar 

  18. Heremans, J. F.: Les globulines sériques du système gamma. Brussels Paris: Arscia et Masson & Cie. 1960.

    Google Scholar 

  19. Hobbs, J. R.: Disturbances of the immunglobulins. In: The scientific basis of medicine Annual Reviews 1966, p. 106. London 1966.

  20. Hobbs, J. R.: Vortrag vor der Royal Academy of Medicine. London, März 1967.

  21. Holmes, A. W., R. B. Capps, andF. Deinhardt: Experimental hepatitis in marmosets. J. clin. Invest.46, 1072 (1967).

    Google Scholar 

  22. Ishizaka, K., T. Ishizaka, andM. M. Hornbrook: Physicochemical properties of reaginic antibody. V. Correlation of reaginic activity with γE-globulin antibody. J. Immunol.97, 840 (1966).

    Google Scholar 

  23. Lee, F. I.: Immunoglobulins in viral hepatitis and active alcoholic liver-disease. Lancet1965 II, 1043.

    Google Scholar 

  24. Mancini, G., A. O. Carbonara, andJ. F. Heremans: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry2, 235 (1965).

    Google Scholar 

  25. McKelvey, E. M., andJ. L. Fahey: Immunoglobulin changes in disease: Quantitation on the basis of heavy polypeptide chains, IgG (γG), IgA (γA), and IgM (γM), and of light polypeptide chains, type K (I) and type L (II). J. clin. Invest.44, 1778 (1965).

    Google Scholar 

  26. Medina, F., e.F. Milazzo: Comportamento delle immunoglobuline nelle malattie infettive. II. Salmonellosi. Atti Accad. med. lombarda21, 23 (1966).

    Google Scholar 

  27. Milazzo, F., F. Medina eC. Banterle: Comportamento delle immunoglobuline nelle malattie infettive. I. Epatite virale. Atti Accad. med. lombarda21, 17 (1966).

    Google Scholar 

  28. Murray, E. S., J. M. O'Connor, andJ. A. Gaon: Serologic studies of primary epidemic typhus and recrudescent typhus II. J. Immunol.94, 734 (1965).

    Google Scholar 

  29. Nossal, G. J. V., A. Szenberg, G. L. Ada, andC. M. Austin: Single cell studies on 19 S antibody production. J. exper. Med.119, 495 (1964).

    Google Scholar 

  30. Ovary, Z.: The structure of various immunoglobulins and their biologie activities. Ann. N.Y. Acad. Sci.129 (I), 776 (1966).

    Google Scholar 

  31. Popper, H., u.F. Schaffner: Die Leber, Struktur und Funktion. Stuttgart: Georg Thieme 1961.

    Google Scholar 

  32. Reddin, J. L., R. K. Anderson, R. Jenness, andW. W. Spink: Significance of 7 S and macrogloculin brucella Agglutinins in human brucellosis. New Engl. J. Med.272, 1263 (1965).

    Google Scholar 

  33. Remington, J. S., andM. J. Miller: 19 S and 7 S anti-toxoplasma antibodies in diagnosis of acute congenital and aquired toxoplasmosis. Proc. Soc. exp. Biol. (N.Y.)121, 357 (1966).

    Google Scholar 

  34. Rowe, D. S., andJ. L. Fahey: A new class of immunoglobulins. II. Normal serum IgD. J. exp. Med.121, 185 (1965).

    Google Scholar 

  35. Schmid, M.: Die chronische Hepatitis. In: Experimentelle Medizin, Pathologie und Klinik, Bd. 18. Berlin-Heidelberg-New York: Springer 1966.

    Google Scholar 

  36. Schwick, H. G.: Immunological methods in clinical chemistry. VI. Int. Kongr. klin. Chem., München 1966 (im Druck).

  37. Schultze, H. E., andJ. F. Heremans: Molecular biology of human proteins with special reference to plasma proteins. Amsterdam-London-New York: Elsevier Publ. Co. 1966.

    Google Scholar 

  38. Smetana, H. F.: Pathologic anatomy of early stages of viral hepatitis. Henry Ford Hosp. Int. Symposium, p. 77. London: J. A. Churchill 1957.

    Google Scholar 

  39. Smith, C. B., J. A. Bellanti, andR. M. Chanock: Serum and nasal antibody to type 1 parainfluenza virus. J. Immunol.99, 133 (1967).

    Google Scholar 

  40. Stiehm, E. R., andH. H. Fudenberg: Serum levels of immunoglobulins in health and disease: A survey. Pediatrics37, 715 (1966).

    Google Scholar 

  41. Störiko, K.: Normal values for 23 different human plasma proteins determined by single radial immunodiffusion. Communication 6th Int. Congr. Clin. Pathol., Rome 1966.

  42. Svehag, S. E., andB. Mandel: The formation and properties of poliovirus-neutralizing antibody. I. 19 S and 7 S antibody formation: Differences in kinetics an antigen dose requirement for induction. J. exp. Med.119, 1 (1964);

    Google Scholar 

  43. II. 19 S and 7 S antibody formation: Differences in antigen dose requirement for sustained synthesis, anamnesis, and sensitivity to x-irrediation. J. exp. Med.119, 21 (1964).

    Google Scholar 

  44. Taliaferro, W. H., andD. W. Talmage: Antibodies in the rabbit with different rates of metabolic decay. J. infect. Dis.99, 21 (1956).

    Google Scholar 

  45. Tobie, J. E., D. C. Abele, S. M. Wolff, P. G. Contacos, andC. B. Evans: Serum immunoglobulin levels in human malaria and their relationship to antibody production. J. Immunol.97, 498 (1966).

    Google Scholar 

  46. Tomasi jr., T. B., andW. A. Tisdale: Serum gamma-globulins in acute and chronic liver diseases. Nature (Lond.)201, 834 (1964).

    Google Scholar 

  47. Uhr, J. W., andM. S. Finkelstein: Antibody formation: IV. Formation of rapidly and slowly sedimenting antibodies and immunological memory to bacteriophage ΦX 174. J. exper. Med.274, 61 (1966).

    Google Scholar 

  48. Wollheim, F. A., andR. C. Williams: Studies on the macroglobulins of human serum. I. Polyclonal immunoglobulin class M (IgM) increase in infectious mononucleosis. New Engl. J. Med.274, 61 (1966).

    Google Scholar 

  49. Wuhrmann, F., u.H. H. Märki: Dysproteinämien und Paraproteinämien. Basel u. Stuttgart: Benno Schwabe & Co. 1963.

    Google Scholar 

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  1. Medizinische Klinik der Medizinischen Hochschule Hannover, Germany

    E. Gleichmann & H. Deicher

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  1. E. Gleichmann
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  2. H. Deicher
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Gleichmann, E., Deicher, H. Quantitative Immunglobulin-Bestimmungen im Serum bei entzündlichen Leberkrankheiten. Klin Wochenschr 46, 171–176 (1968). https://doi.org/10.1007/BF01746229

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