Heart and Vessels

, Volume 7, Issue 1, pp 37–41 | Cite as

Endothelin-induced changes in intracellular calcium concentration in rat vascular smooth muscle cells: Effects of extracellular calcium and atrial natriuretic factor

  • Motoyuki Nakamura
  • Tessa J. Abell
  • A. Mark Richards
  • Hamid Ikram
  • Eric A. Espiner
  • Tim G. Yandle


The purpose of this study was to examine whether different mechanisms might underlie the changes in intracellular calcium concentration ([Ca2+]i) stimulated by high and low concentrations of endothelin, and whether atrial natriuretic factor (ANF) has an inhibitory effect on endothelin-induced [Ca2+]i changes in cultured rat vascular smooth muscle cells (VSMCs). In calcium-replete buffer, cultured monolayers of rat VSMCs superfused with endothelin at a high concentration (10 nM) exhibited a marked transient rise in [Ca2+]i, followed by a sustained elevation, whereas a low concentration of endothelin (0.1 nM) induced a sustained monophasic elevation. When calcium-free buffer was used, 10 nM endothelin induced a transient rise in [Ca2+]i of lesser amplitude, whereas 0.1 nM endothelin did not produce a significant rise. Pretreatment of VSMCs with ANF and cosuperfusion with endothelin failed to inhibit either transient or sustained endothelin-induced changes in [Ca2+]i in calcium-replete buffer.

Key words

Endothelin Atrial natriuretic factor Intracellular calcium concentration Vascular smooth muscle cells 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Motoyuki Nakamura
    • 1
  • Tessa J. Abell
    • 1
  • A. Mark Richards
    • 1
  • Hamid Ikram
    • 1
  • Eric A. Espiner
    • 1
  • Tim G. Yandle
    • 1
  1. 1.Departments of Cardiology and EndocrinologyThe Princess Margaret HospitalChristchurchNew Zealand

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