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Zum Nachweis intrinsic factor-spezifischer Antikörper mit Hilfe der Gelfiltration

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Zusammenfassung

Die Methode der Gelfiltration wurde zum Nachweis von intrinsic-factor-spezifischen Antikörpern verschiedenen Typs eingesetzt. Im Rahmen einer Untersuchungsreihe an 15 Patienten mit perniziöser Anämie und 4 weiteren Kranken mit intrinsic factor-spezifischen Antikörpern erwies sich die in ihrer Handhabung einfache Methode als ausreichend empfindlich und zuverlässig. Im Methodenvergleich unter Anwendung der Charcoal-Technik bzw. der immunologischen Kopräcipitation fand sich eine gute Übereinstimmung der mit den verschiedenen Methoden gewonnenen Ergebnisse.

Summary

For evaluation of antibodies to intrinsic factor of the blocking and non blocking type in patients with pernicious anemia the method of gel filtration with Sephadex G25 and G200 was used. Gel filtration proved to be a simple, reliable and sensitive procedure to detect antibodies to intrinsic factor. When this method was compared with the char coal technic or to immunological coprecipitation a good correlation of results was obtained.

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Authors and Affiliations

  1. Medizinische Universitätsklinik Tübingen, Germany

    W. Hartl & E. Genth

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  1. W. Hartl
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  2. E. Genth
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Herrn Prof. Dr.H. E. Bock zum 65. Geburtstag.

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Hartl, W., Genth, E. Zum Nachweis intrinsic factor-spezifischer Antikörper mit Hilfe der Gelfiltration. Klin Wochenschr 47, 89–93 (1969). https://doi.org/10.1007/BF01745773

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