Molecular and Cellular Biochemistry

, Volume 11, Issue 3, pp 137–147 | Cite as

Cell surface mannosyl transferase activity in the liver of embryonic chick

A Mannose containing Glycolipid as Intermediate in Glycoprotein Synthesis
  • Dieter Arnold
  • Erika Hommel
  • Hans-Jörg Risse
General and Review Articles a. general articles

Summary

Trypsin dissociated intact cells of embryonic chick liver catalyze the transfer of mannose from exogenous GDP-mannose to glycoproteinin vitro. In cells of 8 day old embryos a surface bound mannosyltransferase-system forms several mannose containing isoprenoid-lipids in a primary step. One of these compounds serves as á substrate in the highly specific second step of the overall reaction, the transfer of mannose to glycoprotein. The isoprenoic intermediate has been isolated and its effectivity as substrate for the incorporation of mannose into glycoprotein has been examined.

Keywords

Cell Surface Trypsin Mannose Intact Cell Transferase Activity 

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Copyright information

© Dr. W. Junk b.v. Publishers 1976

Authors and Affiliations

  • Dieter Arnold
    • 1
  • Erika Hommel
    • 1
  • Hans-Jörg Risse
    • 1
  1. 1.Institut für Molekularbiologie und BiochemieFreien Universität BerlinGermany

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