Cancer Immunology, Immunotherapy

, Volume 30, Issue 5, pp 257–261 | Cite as

Altered biodistribution of indium-111-labeled monoclonal antibody 96.5 to tumors and normal tissues of nude mice bearing human melanoma xenografts in visceral organs

  • David R. McCready
  • Janet Price
  • Charles M. Balch
  • James L. Murray
Original articles

Summary

Human melanoma xenografts were produced in the subcutis, kidney, cecum and liver of different nude mice. An111In-labeled anti-(human melanoma) monoclonal antibody (96.5) or an111In-labeled nonspecific control monoclonal antibody (ZCE-025) was injected intravenously in separate groups of mice. Radioactive antibody accumulation was measured in tumor, blood, viscera, and carcasses. mAb 96.5 targeted specifically to tumor tissue regardless of site of growth. Tumors in the liver exhibited significantly (P <0.05) higher tumor-to-blood ratios (45±6, mean ±SEM) than xenografts at other visceral organs, the lowest value being found for subcutaneous melanoma (2.6±0.5). The differences in tumor-to-blood ratio were due to significant alterations of antibody biodistribution, since the actual antibody concentration in the different tumor sites was similar. The percentage of recovered anti-melanoma antibody per milliliter of blood in mice with visceral lesions (4.6±1.1%/ml) was significantly lower than that found in mice with subcutaneous tumors (9.5±1.4%/ml,P <0.05). Moreover, significantly higher levels (18.2±3.2%/g, 31.0±5.1%/g, respectively) of the melanoma mAb 96.5 were found in normal liver and spleen tissue recovered from mice with visceral tumors as compared to tissue from mice with subcutaneous tumors (9.2±0.9%/g, 13.5±1.9%/g, respectively;P <0.05). These results demonstrate that the presence of visceral tumor can significantly affect tumor-to-blood ratios, blood levels, and biodistribution of111In-labeled mAb 96.5.

Keywords

Melanoma Monoclonal Antibody Nude Mouse Human Melanoma Antibody Concentration 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • David R. McCready
    • 1
  • Janet Price
    • 2
  • Charles M. Balch
    • 1
  • James L. Murray
    • 3
  1. 1.Department of General SurgeryM. D. Anderson Cancer CenterHoustonUSA
  2. 2.Department of Cell BiologyM. D. Anderson Cancer CenterHoustonUSA
  3. 3.Department of Clinical Immunology and Biological TherapyThe University of Texas M. D. Anderson Cancer CenterHoustonUSA

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