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Application of isoelectric focusing for studies of major histocompatibility complex class I antigen expression on human carcinomas and sarcomas

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Summary

By one-dimension isoelectric focusing we analysed the major histocompatibility complex class I antigen expression on human tumours. Blood lymphocytes of the patients, processed in parallel, served as a basis for comparison. The prerequisite for the analysis is the preparation of metabolically active tumour cell suspensions devoid of significant leucocyte contamination. The method was found to be suitable for study of the expression of HLA alleles on ex vivo tumour cells and allowed the detection of changes imposed by in vitro treatment with interferon γ and tumour necrosis factor α.

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References

  1. Andersson ML, Stam NJ, Klein G, Ploegh HL, Masucci Mg (1990) Aberrant expression of HLA class-I antigens in Burkitt lymphoma cells Int J Cancer (in press)

  2. Bahn AK, DesMarias CL (1983) Immunohistological characterisation of major histocompatibility antigens and inflammatory cellular infiltrate in human breast cancer. J Natl Cancer Inst 71: 507

    Google Scholar 

  3. Björkmann PJ, Saper MA, Samraoui B, Bennett WS, Strominger JL, Wiley DC (1987) The foreign antigen binding site and T cell recognition regions of class-I histocompatibility antigens. Nature 329: 512

    Google Scholar 

  4. Böyum A (1974) Separation of blood leukocytes, granulocytes and lymphocytes. Tissue Antigens 4: 269

    Google Scholar 

  5. Doyle A, Martin WJ, Gazdar A, Carney D, Martin SE, Linnoila I, Cuttita F, Mulshine J, Bunn P, Minna J (1985) Markedly decreased expression of class-I histocompatibility antigens, protein and mRNA in human small cell lung cancer. J Exp Med 161: 1135

    Google Scholar 

  6. Edidin M, (1983) MHC antigens and non-immune functions, Immunol Today 4: 269

    Google Scholar 

  7. Festenstein H (1987) The biological consequences of altered MHC expression on tumors. Br Med Bull 43: 217

    Google Scholar 

  8. Hämmerling GJ, Klar D, Katzaw S, Segal S, Feldman M, Wallich R, Hämmerling A (1986) Manipulation of metastasis and tumor growth by transfection with histocompatibility class-I antigens. J Immunogenet 13: 153

    Google Scholar 

  9. Hämmerling GJ, Klar D, Pulm W, Momburg F, Moldenhauer G (1987) The influence of major histocompatibility complex class-I antigens on tumor growth and metastasis. Biochim Biophys Acta 907: 245

    Google Scholar 

  10. Julius MH, Simpson E, Herzenberg LA (1974) Rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol 3: 645

    Google Scholar 

  11. Momburg F, Möller P, Moldenhauer C, Hämmerling GJ (1986) Loss of HLA-ABC in colorectal carcinoma is related to the degree of de-differentiation. J Immunogene 13: 195

    Google Scholar 

  12. Momburg F, Ziegler A, Harppprecht J, Möller P, Moldenhauer G, Hämmerling GJ (1989) Selective loss of HLA-A or HLA-B antigen expression in colon carcinoma. J Immunol 142: 325

    Google Scholar 

  13. Natali Pg, Nicortra MR, Bigotti A, Venturo I, Marcenaro L, Giacomini P, Russo C (1989) Selective changes in expression of HLA class-I polymorphic determinants in human solid tumors. Proc Natl Acad Sci USA 86: 6719

    Google Scholar 

  14. Neefjes JJ, Breur-Vriesendorp BS, van Seventer GA, Ivanyi P, Ploegh HL (1986) An improved biochemical method for the analysis of HLA-Class-I antigens. Definition of new HLA-class-I subtypes. Hum Immunol 16: 169

    Google Scholar 

  15. Parham P, Barnstable CJ, Bodmer WF (1979) Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C-antigens. J Immunol 123: 324

    Google Scholar 

  16. Perez M, Cabrera T, Lopez-Nevot MA, Gomez M, Peran F, Ruiz-Cabello F, Garrido F (1986) Heterogenicity of the expression of class-I and class-II HLA antigens in human breast carcinoma. J Immunogenet 13: 247

    Google Scholar 

  17. Ploegh HL, Orr HT, Strominger JL. (1981) Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class-I molecules. Cell 24: 287

    Google Scholar 

  18. Rees RC, Buckle A-M, Gelsthorpe K, James V, Potter CW, Rogers K, Jacob G (1988) Loss of polymorphic A and B locus HLA antigens in colon carcinoma. Br J Cancer 57: 374

    Google Scholar 

  19. Smith MEF, Bodmer WF, Bodmer JG (1988) Selective loss of HLA-A,B,C locus products in colorectal adenocarcinoma. Lancet I, 823

    Google Scholar 

  20. Smith MEF, Marsh SGE, Bodmer JG, Geisthorpe K, Bodmer WF, (1989) Loss of HLA-A,B,C allele products and lymphocyte function-associated antigen 3 in colorectal neoplasia. Proc Natl Acad Sci USA 86: 5557

    Google Scholar 

  21. Townsend ARM, McMichael AJ, Carter NP, J. A. Huddleston JA and Brownlee GG. (1984) Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell 39: 13

    Google Scholar 

  22. Vánky F, (1986) Membrane structures involved in auto-tumor recognition. Biochim Biophys Acta 865

  23. Vánky F, Roberts T, Klein E, Willems J (1987) Auto-tumor immunity in patients with solid tumors: participation of CD3 complex and MHC class-I antigens in the lytic interaction. Immunol Lett 16: 21

    Google Scholar 

  24. Vánky F, Stuber G, Willems J, Sjöwall K, Larsson B, Böök K, Ivert T, Péterffy A, Klein E (1988) Importance of MHC antigen expression on solid tumors in the in vitro interaction with autologous blood lymphocytes. Cancer Immunol Immunother 27: 213

    Google Scholar 

  25. Vánky F, Stuber G, Rotstein S, Klein E (1989) Auto-tumor recognition following in vitro induction of MHC antigen expression on solid tumors: stimulation of lymphocytes and generation of cytotoxicity against the original MHC-antigen-negative tumor cells. Cancer Immunol Immunother 28: 17

    Google Scholar 

  26. Vánky F, Wang P, Patarroyo M, Klein E (1990) Expression of the adhesion molecule ICAM-1 and major histocompatibility complex class-I antigens on human tumor cells is required for their interaction with autologous lymphocytes in vitro. Cancer Immunol Immunother 31: 19

    Google Scholar 

  27. Whitwell HL, Hughes HPA, Moore M, Ahmed A (1984) Expression of major histocompatibility antigens and leukocyte infiltration in benign and malignant human breast disease. Br J Cancer 49: 161

    Google Scholar 

  28. Yang SY (1989) Joint reports: HLA-class-I polymorphism detected by one-dimensional isoelectric focusing. In: Bo Dupont (ed), Immunobiology of HLA, vol 1. Springer Berlin, Heidelberg, New York, pp 309–354

    Google Scholar 

  29. Yewdell JN, Bennink JR, Smith L, Moss B (1985) Influenza A virus nucleoprotein is a major target antigen for cross reactive anti-influenza A virus cytotoxic T lymphocyts. Proc Natl Acad Sci USA 82: 1785

    Google Scholar 

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Authors and Affiliations

  1. Department of Tumor Biology, Karolinska Institute, Box 60 400, S-104 01, Stockholm, 60 500, Sweden

    P. Wang, F. Vánky & E. Klein

  2. Department of Experimental Oncology, Radiumhemmet, Karolinska Hospital, S-104 01, Stockholm 60 500, Sweden

    P. Wang & F. Vánky

Authors
  1. P. Wang
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  2. F. Vánky
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  3. E. Klein
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Wang, P., Vánky, F. & Klein, E. Application of isoelectric focusing for studies of major histocompatibility complex class I antigen expression on human carcinomas and sarcomas. Cancer Immunol Immunother 33, 65–69 (1991). https://doi.org/10.1007/BF01742531

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  • DOI: https://doi.org/10.1007/BF01742531

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