Cancer Immunology, Immunotherapy

, Volume 33, Issue 1, pp 45–49 | Cite as

Interleukin-4 augments the cytotoxic capacity of lymphocytes and monocytes in antibody-dependent cellular cytotoxicity

  • Peter Wersäll
  • Giuseppe Masucci
  • Håkan Mellstedt
Original articles


Human peripheral blood mononuclear cells (lymphocytes and monocytes) (PBMC) were preincubated for 0–24 h with human recombinant interleukin-4 (IL-4) and used as effector cells in an 18 h antibody-dependent cellular cytotoxicity (ADCC) assay with mAb 17-1A (mouse IgG2A) against SW948 (a human colorectal carcinoma cell line). A statistically significant increase in the lytic capability was noted after 2–24 h of preactivation. IL-4 at 1 ng/ml induced the highest cell lysis while higher and lower concentrations were inferior or had no effect at all. Preactivation for 24 h induced a more effective lytic cell population than 2 h prestimulation: 63 LU (lytic units)/106 cells vs 42 LU/106 cells. Pretreatment with 1 ng/ml IL-4 for 2 h induced a statistically significant increase in the ADCC activity of PBMC (P <0.05), of monocytes (P <0.01) and E-rosette-negative cells (natural killer cells) (P <0.05) compared to non-activated cells. IL-4 did not induce lymphokine-activated killer activity of PBMC against SW948. The spontaneous cytotoxicity against K562 was, however, increased after stimulation with 1 ng/ml IL-4 for 2 h of E-rosette-negative non-adherent cells.

Key words

IL-4 ADCC Monoclonal antibodies 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Peter Wersäll
    • 1
  • Giuseppe Masucci
    • 1
  • Håkan Mellstedt
    • 1
  1. 1.Department of Oncology (Radiumhemmet) and Immunologic Research LaboratoryKarolinska HospitalStockholmSweden

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