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Lymphokine mRNA profile and functional analysis of a human CD4+ clone with unique antitumor specificity isolated from renal cell carcinoma ascitic fluid

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Summary

We here describe the isolation, characterization, profile of lymphokine expression and T-cell-receptor gene rearrangment pattern of 444P.3, a CD3+ CD4+ CD8− 4B4+ interleukin-2 (IL-2)-dependent clone derived from the malignant ascites of a patient with renal cell cancer. The 444P.3 clone exhibited unique antitumor specificity between days 45 and 84 in culture and then lost its lytic, but not its proliferative, capacity. To our knowledge this is the first description of a specific antitumor reaction in a patient with renal cell cancer against autologous tumor. IL-2-expanded 444P.3 cells, tested on day 104 in culture, expressed mRNA for tumor necrosis factor (TNF), IL-2 and tumor growth factor β (TGF-β) but not for IL-1, lymphotoxin or granulocyte/macrophage-colony stimulating factor (GM-CSF). The parental noncloned population expressed mRNA for TNF, lymphotoxin, GM-CSF and TGF-β but not for IL-1β or IL-2. Analysis of established human T cell clones should include profiles of lymphokine secretion in addition to growth and proliferation patterns, antitumor activity and surface phenotype. Such characterization of clones may provide a better understanding of the immunoregulatory role and functional potential of various T cell subsets involved in human antitumor reactivity.

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Authors and Affiliations

  1. Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

    Arie Belldegrun, Atan Kasid, Michael Uppenkamp & Steven A. Rosenberg

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  1. Arie Belldegrun
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  2. Atan Kasid
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  3. Michael Uppenkamp
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  4. Steven A. Rosenberg
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Belldegrun, A., Kasid, A., Uppenkamp, M. et al. Lymphokine mRNA profile and functional analysis of a human CD4+ clone with unique antitumor specificity isolated from renal cell carcinoma ascitic fluid. Cancer Immunol Immunother 31, 1–10 (1990). https://doi.org/10.1007/BF01742489

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  • DOI: https://doi.org/10.1007/BF01742489

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