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Generation of T cell clones binding F(ab′)2 fragments of the idiotypic immunoglobulin in patients with monoclonal gammopathy

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Summary

Lymphocytes from two patients with multiple myeloma stage I and one patient with monoclonal gammopathy of undetermined significance were found to proliferate specifically in response to low concentrations of F(ab′)2 fragments of the autologous M component. T cell clones isolated from repeatedly stimulated cultures bound specifically the autologous idiotype and proliferated after addition of soluble idiotype and exogenous interleukin-2. The majority of clones were CD8+ and showed negligible staining for CD4. Idiotype-binding clones could not be isolated from cultures of lymphocytes from a healthy control stimulated under the same conditions. The study provides support for the existence of idiotype-reactive T cells in monoclonal gammopathies. Such cells might have a regulatory role on the tumour cell clone and may be important for a future therapeutic approach.

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Authors and Affiliations

  1. Department of Oncology (Radiumhemmet), Department of Medicine, Immunological Research Lab., Karolinska Hospital, S-104 01, Stockholm, Sweden

    Anders Österborg, Susanne Bergenbrant, Göran Holm, Ann-Kari Lefvert & Håkan Mellstedt

  2. Department of Tumour Biology, Karolinska Institute, Stockholm, Sweden

    Maria Masucci

Authors
  1. Anders Österborg
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  2. Maria Masucci
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  3. Susanne Bergenbrant
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  4. Göran Holm
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  5. Ann-Kari Lefvert
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  6. Håkan Mellstedt
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Österborg, A., Masucci, M., Bergenbrant, S. et al. Generation of T cell clones binding F(ab′)2 fragments of the idiotypic immunoglobulin in patients with monoclonal gammopathy. Cancer Immunol Immunother 34, 157–162 (1991). https://doi.org/10.1007/BF01742306

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  • DOI: https://doi.org/10.1007/BF01742306

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