Hydrophobic surface properties of erythrocytes studied by affinity partition in aqueous two-phase systems
Erythrocytes from various species have been partitioned in aqueous two-phase systems consisting of water, dextran, poly-(ethylene glycol), salt and buffer. The terminal hydroxyl groups of the latter polymer were esterified with palmitic, oleic, linoleic and linolenic acids, as well as with deoxycholic acid. In a two-phase system containing unesterified poly(ethylene glycol) the erythrocytes are exclusively in the dextran-rich lower phase. When the poly(ethylene glycol) is esterified the red blood cells collect at the interface and/or in the poly(ethylene glycol)-rich upper phase depending on the type and concentration of esterified acid. Palmitate ester is most effective in increasing the affinity of the cells for the upper phase, followed by oleate, linolate, linolenate, and deoxycholate esters. The partition behaviour of erythrocytes from various species differs considerably. Two groups can be distinguished: one consisting of erythrocytes from dog, guinea pig and rat, the other from human, sheep and rabbit. This division can be correlated to the content of sphingomyelin and phosphatidyl choline in the erythrocyte membranes.
KeywordsLinolenic Acid Choline Dextran Oleate Phosphatidyl Choline
Unable to display preview. Download preview PDF.
- 1.Shanbhag, V. P. and Johansson, G., 1974. Biochem. Biophys. Res. Commun. 61, 1141–1146.Google Scholar
- 2.Takerkart, G., Segard, E. and Monsigny, M., 1974. FEBS Letters, 42, 218–220.Google Scholar
- 3.Flanagan, S. D. and Barondes, S. H., 1975. J. Biol. Chem., 250, 1484–1489.Google Scholar
- 4.Flanagan, S. D., Taylor, P. and Barondes, S. H., 1975. Nature 254, 441–443.Google Scholar
- 5.Albertsson, P. Å., 1971. Partition of Cell Particles and Macromolecules, 2nd edn., Almqvist & Wilksell, Uppsala, Sweden.Google Scholar
- 6.Vowinkel, E., 1967. Chem. Ber. 100, 16–22.Google Scholar
- 7.Hanus, J., 1901. Z. Untersuch Nahr. Genussm. 4, 913–920.Google Scholar
- 8.Chapman, D., 1968. Biological Membranes, pp. 24–25, Academic Press, London.Google Scholar
- 9.Bretscher, M., 1973. Science 181, 622–629.Google Scholar